Scientific Paper 4-10-13 Determining the Unknown Genotype of Corn Plants of the Zea mays Species from the Phenotypes of Offspring Produced Abstract No one particular organism is an exact replica of another. Diversity in the world is key for future generations to adapt to the fast changing world. This lab observed the corn plant of the Zea mays species to determine the genotype for the gene of tall versus dwarf in unknown parent corn plants by observing the seedlings produced. It was hypothesized
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bacteria can use citrate as a source of carbon. To test if the unknown bacteria uses citrate as a source of carbon‚ Simmon’s citrate agar was used as the medium on which the bacteria was grown. The Simmon’s citrate agar consists of sodium citrate as the source of carbon‚ ammonium dihydrogen phosphate as the source of nitrogen along with pH indicator such as bromothymol blue. Procedure: The Citratase activity was detected by inoculating the unknown bacteria on the slant surface of Simmon’s citrate agar
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Microbiology Laboratory Report Identification of Unknown Bacteria 03/10/05- 04/01/05 Authors: Richard Hendricks‚ Jessica Prebish; NMU Abstract: Broth culture 16 was randomly selected by our group and subjected to qualitative tests for taxonomic identification. The culture did appear homogenous throughout the testing period and is currently retained by Northern Michigan University’s department of Microbiology. We suggest that culture 16 is an example of Escherichia coli. Background: Techniques
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Identification of Unknown Bacteria 6O BIO 251-7H1 Gram Stain Summary: Out of 28 Unknown microorganisms‚ slant 6O was randomly selected‚ and subjected to testing in order to identify it’s species. Seventeen of the unknown bacteria were classified as Gram-negative bacilli: Corynebacterium pseudodiphtheriticum‚ Enterobacter aerogenes‚ Eschirichia coli‚ Flavobacter capsulatum‚ Helicobacter pylori‚ Klebsiella pneumonia‚ Klebsiella oxytoca‚ Proteus hauseri‚ Proteus mirabilis‚ Proteus vulgaris‚ Pseudomonas
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Page I - Cover sheet In the middle f the page give name and number of your microorganism In the right lower corner provide - your name - Lab section number (Biol 108-005) - Date submitted ( 4/18/2013) - the unknown tube # is 5 Page II table of result - This page will have your table of results include the following information - Name of the test - Medium used - Indicator used - your results Part III - All the test done As many pages as needed to do a complete job. in this section
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Gram Positive Unknown: “Enterococcus faecalis” Family: enterococcaceae Genus: enterococcus Species: faecalis Gram + Oxygen class: facultative anaerobe Temperature class: mesophile – they can grow in the range of 10 °C - 45 °C pH class: can grow at a pH range of (4.6 – 9.9) with the optimum at 7.5 Enterococci can survive very harsh environments including extreme alkaline pH 9.6 and salt concentrations (basic). Environment: They can
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The aseptically inoculate nutrient broth tubes A and B did not have any microbes inoculated into the tubes so it should not have been cloudy and that was what was seen. Tube C should have been cloudy because it had E. coli inoculated into the nutrient broth and that was also seen. A simple streak was also performed and it looks has if the loop went over previous streaks which should not have been done. A complex streak was also done. No colonies grew towards the end of the streak‚ this could be due
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then observed protein catabolism in Entrobacter aerogenes and Proteus vulgaris. We inoculated tubes with each bacteria‚ one containing a nutrient gelatin and one a urea agar medium and incubated the tubes‚ at room temperature and observed the growth and the color to determine whether hydrolysis took place. (Table 3) * To observe protein catabolism we also inoculated one tube with Enterobacter aerogenes and the another Proteus vulgaris‚ allowed them to incubate and observed the growth and color
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The tube that contained my unknown material was tube number twenty. I did a gram stain‚ oxidase test‚ and used three different medias to narrow down and identify my unknown organism. The first step I took in identifying was a gram stain. This gram stain would reveal whether my organism is gram positive or gram negative which would narrow my choices from fifteen options down to six. After completing the gram stain‚ my unknown was the color pink. This revealed my unknown as being gram negative. I
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sulfide Test | Urease Test | CatalaseTest | Salmonellaenterica | (-)Red color | (+)YellowColor | (-)No red ring | (+)RedRing | (-)No pink | (+)Blue With growth | (+)Black PrecipitateVery motile | (-)No change in color | (+) | The unknown organism (#11) was Salmonella enterica‚ which is a rod-shaped‚ Gram negative organism. They are non-spore forming; very motile .These organisms are chemoorganotrophs (an organism that obtains energy from the oxidation of reduced organic compounds)
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