Gram Positive Unknown: “Enterococcus faecalis” Family: enterococcaceae Genus: enterococcus Species: faecalis Gram + Oxygen class: facultative anaerobe Temperature class: mesophile – they can grow in the range of 10 °C - 45 °C pH class: can grow at a pH range of (4.6 – 9.9) with the optimum at 7.5 Enterococci can survive very harsh environments including extreme alkaline pH 9.6 and salt concentrations (basic). Environment: They can
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The aseptically inoculate nutrient broth tubes A and B did not have any microbes inoculated into the tubes so it should not have been cloudy and that was what was seen. Tube C should have been cloudy because it had E. coli inoculated into the nutrient broth and that was also seen. A simple streak was also performed and it looks has if the loop went over previous streaks which should not have been done. A complex streak was also done. No colonies grew towards the end of the streak‚ this could be due
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then observed protein catabolism in Entrobacter aerogenes and Proteus vulgaris. We inoculated tubes with each bacteria‚ one containing a nutrient gelatin and one a urea agar medium and incubated the tubes‚ at room temperature and observed the growth and the color to determine whether hydrolysis took place. (Table 3) * To observe protein catabolism we also inoculated one tube with Enterobacter aerogenes and the another Proteus vulgaris‚ allowed them to incubate and observed the growth and color
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The tube that contained my unknown material was tube number twenty. I did a gram stain‚ oxidase test‚ and used three different medias to narrow down and identify my unknown organism. The first step I took in identifying was a gram stain. This gram stain would reveal whether my organism is gram positive or gram negative which would narrow my choices from fifteen options down to six. After completing the gram stain‚ my unknown was the color pink. This revealed my unknown as being gram negative. I
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sulfide Test | Urease Test | CatalaseTest | Salmonellaenterica | (-)Red color | (+)YellowColor | (-)No red ring | (+)RedRing | (-)No pink | (+)Blue With growth | (+)Black PrecipitateVery motile | (-)No change in color | (+) | The unknown organism (#11) was Salmonella enterica‚ which is a rod-shaped‚ Gram negative organism. They are non-spore forming; very motile .These organisms are chemoorganotrophs (an organism that obtains energy from the oxidation of reduced organic compounds)
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Pseudomonas aeruginosa form irregular colony‚ reaised elevation‚ lobate margin‚ and wet texture. Other bacteria‚ that are Serratia marcescens‚ Proteus vulgaris‚ and Klebsiella aerogenes form circular colony. For S. marcescens and K. aerogenes they have entire margin while P. vulgaris form even margin. S. marcescens form umbonate elevatiob and mucoid texture. While P. vulgaris form raised elevation and smooth texture. Klebsiella so form raised elevation and dry texture. For triple sugar ion is used to differentiate
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25922 + red Proteus mirabilis 12453 + colourless Salmonella typhimurium 14028 + colourless Streptococcus faecalis 29212 - or partial Uses Acting as a visual ph indicator‚ the agar distinguishes those Gram-negative bacteria that can ferment the sugar lactose (Lac+) from those that cannot (Lac-). This medium is also known as an "indicator medium" and a "low selective medium". Absence of electrolytes serves to inhibit swarming by Proteus species. Lac+
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Hydrolysis Report Bile Esculin Hydrolysis‚ Starch Hydrolysis‚ UreaHydrolysis‚ Casein Hydrolysis Hydrolysis Report Introduction Hydrolysis is a chemical reaction that uses water to split complex molecules. The water molecule H2O is split in the mechanism of hydrolysis‚ hydrogen cations and hydroxide anions. When the enzyme catalyzes its reaction inside the cell‚ it is referred to as intracellular hydrolases. When the enzymes secreted from the organism to catalyze reactions outside the cell‚ it
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Gram Stain: The important part of this experiment is being able to determine a bacterium based on its cell wall structure. It also helps indentify if the unknown organism is a Gram positive or Gram negative. This is the initial step that must be taken before any other lab procedure may continue on to ensure the purity is present‚ the arrangement of the cells‚ and the shape the cell has. The staining of the cell starts off with using the primary stain‚ Crystal Violet which is a purple color‚ to begin
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Biochemical Test – Enterobacteriaceae Bacterial spp incapable of fermenting glucose cannot utilize lactose. 2 enzymes necessary for a bacterium to take up lactose: A. β-galactoside permease – facilitates the entry of lactose molecules to bac cell wall B. β-galactosidase – breaks down lactose into β-D glucose and β-D galacatose LF possess both enzymes NLF do not possess β-galactosidase LLF do not possess β-galactoside permease Glucose fermenters only (true enteric pathogen)
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