University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Three test tubes were filled with 5 mL of A‚ B‚ and C‚ respectively. 10 drops of mammal blood was added to each of these and mixed. The transparency and translucency of these tubes was tested. The solutions in the opaque tubes were placed on a slide with a drop of blood and observed under a microscope and
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starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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A. Avril Crayfish Lab Report November 9‚ 2012 Dr. Marvin Results: Figure 1. Firing Rate of Tonic Receptor in Response to Stretch. The correlation between Firing Rate and Stretch of the slow adapting crayfish receptor for four different sets of data is represented in this figure. The recordings are taken at stretches of 2‚ 4‚ 6‚ 8‚ and 10 mm of the crayfish tail. The best fit lines for the different sets of data are as follows: Ali and Emily- Linear best fit line‚ Dave and Laura- Exponential
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Ø 29 & 30 Worksheets 16. Garbage In‚ Garbage Out Ø 31 & 32 Worksheets (ii) 1 FOOD : WHERE DOES IT COME FROM WORKSHEET-1 SUMMATIVE ASSESSMENT Objective type questions Choose the correct option : 1. (a) 2. (d) 3. (c) 4. (c) 5. (b) 6. (b) 7. (b) 8. (c) 9. (a) 10. (d). Answer the Following : Ans 1 : We require energy to perform different activities. This energy is obtained from the food we eat which is oxidized in the cells to release energy. Ans 2 : Two ingredients are needed
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Sterling Hayden AP Biology Mrs. Proehl 24 November 2014 I. Title Effect of Differing pH‚ Temperature‚ and Enzyme Concentration on Catalase Reaction Rate II. Introduction Enzymes are used to increase the rate of specific reactions in the body. Catalase‚ a specific enzyme‚ speeds the breakdown of hydrogen peroxide‚ a toxic chemical produced by cells in the body‚ into water and oxygen (Cain and others‚ 2010). The oxygen can be observed as bubbles coming from the reaction site. Catalase is found in many living tissues of organisms
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Introduction: The purpose of this lab is to see how the colour of an apple would change (browning) over time‚ when placed in different conditions. When referring to the term “browning”‚ it is meant to imply the change of colour that occurs inside the apple‚ giving an appearance that is distasteful (Di Guardo et al.‚ 2013). Specifically‚ within this lab the apple was cut into 4 pieces with 3 pieces being placed on a weigh boat‚ and a 4th piece placed in a beaker of water. These weigh boats were
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amount of time that was given for the Daphnia to absorb the nicotine and the concentration of nicotine given to the
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A Project Report On C Programming Submitted TO‚ Submitted By‚ __________________ __________________ Grade: XII Date of Submission: __/__/____ Acknowledgement I am very thankful to everyone who all supported me‚ for I have completed my project effectively and moreover‚ on time. I am equally grateful to my computer teacher. He gave me moral support and guided me in different matters regarding the topic. He has been very kind and
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Oscillators & Oscilloscope Morgan Dennis @02514008 Partners: Brittany Smith‚ Michelle Trang‚ James Anderson‚ Madison Shaw OBJECTIVE: We are going to study the features and operation of the oscilloscope. We will use the instrument to measure the frequency and amplitude for various sources. Ultimately‚ the oscilloscope will display the Lissajous figures. APPARATUS: The major apparatus used include; Oscilloscope‚ two audio oscillators‚ battery and connectors. THEORY: The oscilloscope
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