Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
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Preliminary Unknown Report Alexis Grettenberger 800503431 Katie Young Alexis Grettenberger Biol 311 Lab 11/21/14 Preliminary Unknown Report Purpose: The purpose of this experiment was to identify a specific unknown bacterium that was isolated from a multitude of bacteria in a sample of contaminated dirt by running it through a series of identification techniques. Some of these techniques were simple staining‚ gram staining‚ and isolation techniques. The hypothesis of this experiment was that
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Introduction In the amylase lab‚ it was being tested if amylase‚ an enzyme found in saliva‚ would be denatured by being put in an acid or high temperatures. This lab is about denaturing amylase. It is tested by exposing it to pH and temperature changes. It is then mixed with Benedict’s solution‚ is a solution that changes color from blue to reddish brown when maltose is present. Amylase breaks starch into maltose‚ so is the amylase isn’t denatured‚ it should change colors. Amylase is an enzyme
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Osmosis The purpose of this lab is to study how membranes of plant and animal cells react when exposed to different solutions. The first experiment involves purple onion skin and the second involves rat blood in various solutions. I needed to understand certain terms before preforming this lab to be able to efficiently explain what is happening to the cells. Diffusion is the tendency of a substance to move down its concentration gradient from a more concentrated to a less concentrated area.
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100 ml of artificial pond water. There were 8 cups in each treatment‚ high density had 100 thali‚ medium density had 50 thali‚ and low density had 10 thali. The cups were randomly placed on a tray. The thali were counted and then rearranged every Monday‚ Wednesday‚ Friday for 7 total changes. Water levels were topped off each visit. The cups were rotated middle to sides and front row to back row. To dispose of the Duckweed‚ we poured them down the drain with plenty of water. A two-way ANOVA was run
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Data Analysis: 1. What was the temperature change for the water in the calorimeter (ΔTwater)? 3°C 2. What was the temperature change for the metal sample (ΔTmetal)? Assume the intial temperature of the metal was the same as the temperature of the water it was boiled in. 70°C 3. What was the specific heat of water in J/g°C? 4.186 J/g°C 4. Using the following equation‚ your answers to questions 1-3‚ and your data table‚ determine the specific heat of the metal sample you tested. 5
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about Mount Kilauea . The volcano Mount Kilauea is located in the state of Hawaii and it can be found on Hawaii ’s biggest island which is Mauna Loa. In the Hawaiian language the name Kilauea means “spewing” or “much spreading” because of the mountain’s constant outpouring of lava (Wikipedia). Mount Kilauea has been expelling lava on a consistent basis since January of 1983. With this in mind‚ it makes Mount Kilauea the most active volcano on the planet. Also because of Mount Kilauea
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Introduction: The purpose of this lab is to see how the colour of an apple would change (browning) over time‚ when placed in different conditions. When referring to the term “browning”‚ it is meant to imply the change of colour that occurs inside the apple‚ giving an appearance that is distasteful (Di Guardo et al.‚ 2013). Specifically‚ within this lab the apple was cut into 4 pieces with 3 pieces being placed on a weigh boat‚ and a 4th piece placed in a beaker of water. These weigh boats were then set
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Mr. Mihordea Motion of Atoms and Molecules lab summary In this lab the standard heat of combustion of magnesium was calculated by using the calorimeter. Also‚ the point of combustion of magnesium was to see how much heat would be released from fireworks such as sparklers. The concept of this lab was to find the difference in temperature from the beginning to end of combustion of magnesium and calculate the standard heat
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student’s hypothesis as it related to the pH of common solutions Hypothesis The pH of the tested solutions will be in the order of the following according to a pH scale: 1. Lime juice 2. Orange juice 3. Soda 4. Iced Tea 5. Milk 6. Water 7. Soapy water Material Required To facilitate this laboratory exercise‚ the experimenter needs the following: pH strips Sample reservoirs of solutions with varying pH Laboratory Notebook pH paper Background pH stands for potential hydrogen
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