1.3.1 response
Activity 1.3.1: Student Response Sheet
PART A- Restriction Enzymes
Restriction enzymes are a tool that allows us to pinpoint human identity down to single differences in our DNA. Work through the following simulation so you can see these molecular scissors in action.
Find out more about restriction enzymes by viewing the animation and reading the article listed below.
DolanDNALearningCenter: Restriction Enzymes http://www.dnalc.org/ddnalc/resources/restriction.html
Access Excellence Classic Collection: Restriction Enzymes Background Paper http://www.accessexcellence.org/AE/AEC/CC/restriction.php
1. From what organism are restriction enzymes derived? What role do these enzymes play in this natural host?Phages. Enzymes protect phages from viruses.
2. In this lab activity, you are digesting your DNA samples with two specific restriction enzymes- EcoRI and HindIII. How does each of these enzymes get its unique names? EcoRI comes from Escherichia coli RY13 bacteria, while HindII comes from Haemophilusinfluenzae strain Rd
3. Each enzyme recognizes a very specific sequence in DNA.
For example, the enzyme PstI recognizes the sequence:
C T G C A G G A C G T C
The enzyme scans DNA for this sequence and makes a cut as indicated by the arrows.
Visit the list of restriction enzymes found at the bottom of the page on http://en.wikipedia.org/wiki/Restriction_enzymes. Find the unique sequence (restriction site) that is recognized by EcoRI and by HindIII. Write the double-stranded sequence below and draw an arrow between the base pairs to indicate where the enzyme would make its cut.
EcoRI: HindIII:
A-AGCTT
TTCGA-A
G-AATTC
CTTAA-G
4. What do you notice about each restriction site? What does the word palindrome mean? Each site is just revrese order top and bottm. Pallindrome: word spelled the same forward and back.
5.
PART A- Restriction Enzymes
Restriction enzymes are a tool that allows us to pinpoint human identity down to single differences in our DNA. Work through the following simulation so you can see these molecular scissors in action.
Find out more about restriction enzymes by viewing the animation and reading the article listed below.
DolanDNALearningCenter: Restriction Enzymes http://www.dnalc.org/ddnalc/resources/restriction.html
Access Excellence Classic Collection: Restriction Enzymes Background Paper http://www.accessexcellence.org/AE/AEC/CC/restriction.php
1. From what organism are restriction enzymes derived? What role do these enzymes play in this natural host?Phages. Enzymes protect phages from viruses.
2. In this lab activity, you are digesting your DNA samples with two specific restriction enzymes- EcoRI and HindIII. How does each of these enzymes get its unique names? EcoRI comes from Escherichia coli RY13 bacteria, while HindII comes from Haemophilusinfluenzae strain Rd
3. Each enzyme recognizes a very specific sequence in DNA.
For example, the enzyme PstI recognizes the sequence:
C T G C A G G A C G T C
The enzyme scans DNA for this sequence and makes a cut as indicated by the arrows.
Visit the list of restriction enzymes found at the bottom of the page on http://en.wikipedia.org/wiki/Restriction_enzymes. Find the unique sequence (restriction site) that is recognized by EcoRI and by HindIII. Write the double-stranded sequence below and draw an arrow between the base pairs to indicate where the enzyme would make its cut.
EcoRI: HindIII:
A-AGCTT
TTCGA-A
G-AATTC
CTTAA-G
4. What do you notice about each restriction site? What does the word palindrome mean? Each site is just revrese order top and bottm. Pallindrome: word spelled the same forward and back.
5.