5.2.5 Detectors Of UPLC

1.5.2.5 Detectors The detectors used in UPLC should be able to handle very fast scanning methods because half-height peak widths of less than one second are usually obtained with columns packed with 1.7 µm particles. The detector must be able to give high sampling rate adequate to capture enough data points across the peak for an accurate and reproducible integration of analyte peak. The dispersion (volume) of the detector flow cell must be minimal to maintain separation efficiency. Conceptually, the increase in sensitivity for UPLC detection should be 2-3 times more than previous HPLC separations, which is due to the detection technique. ACQUITY Photodiode Array (PDA) and Tunable UV (TUV) are the detectors employed in the UPLC. In these detectors,
It decreases process cycle time and persuade quality of end-product with reduced run time and decreased cost of operation. Through the use of a novel column material of very small particle size, it provides quick analysis and increases sensitivity. It reduces the solvent consumption and raises sample throughput and also supports real-time analysis in step with manufacturing
When the pH of the mobile phase is equivalent to pKa value of analyte, the compound is half ionized. If the pH is 1 unit away from the pKa value of analyte then the change in the extent of ionization is around 90%, If the pH is 2 units away from the pKa value of analyte, the change in the extent of ionization is around 99%, If the pH is 3 units away from the pKa value of analyte, the change in the extent of ionization is around 99.9% etc. Therefore, the rule of thumb called as the 2 pH rule is useful in expecting the extent of ionization. Nearly all the pH related change occurs within ± 2 units of the pKa value. The compound is either ionized or non-ionized at outside this range, and the compound retention time does not change much with