Affects of Ph on Peroxidase Enzyme
The Affects of pH on Peroxidase Enzyme
Claire Bell
Biology 1130
Jazmen Myers
Section 36
Abstract Peroxidase is a turnip enzyme; it is used in the oxidation of hydrogen peroxide to lower activation energy, speeding up the reaction. The activity of peroxidase is highly dependent on its environment and most importantly the pH level. Peroxidase has been the focus of many recent studies and is believed to possibly reduce swelling among other things. We conducted an experiment testing the effect different levels of pH had on the reaction rate of peroxidase. In the experiment we created different solutions all containing hydrogen peroxide, peroxidase, and guaiacol. However each cuvette contained a different pH level, 2,5,7,or 10. Through the experiment we found the activity of peroxidase is highest at its optimal pH level between 6.0 and 7.0 and ceases to react at pH levels 2.5 and lower and between 8.5 and 9.5 and higher. Our data supported the idea that peroxidase is slightly acidic enzyme; however the more acidic pH levels are more effective as inhibitors opposed to the highly basic pH levels.
Introduction
In this experiment we tested the effects of the environmental parameter pH on peroxidase activity. We performed this experiment to observe and analyze the effect pH has on peroxidase activity and find the optimal pH level for peroxidase, as well as the ceasing point in the reaction. Parameters are characteristics that can alter an experiment. The parameter we used in this experiment was pH. The pH scale ranges from levels 0 to 14, 0 being the most acidic, and 14 being the most basic. An enzyme is a biological catalyst made up of proteins. Enzymes speed up reactions by bringing reactants close together and lowering the activation energy. Enzymes are extremely specific to their substrates. A substrate is material where an organism lives or the surface upon which an organism grows or is attached. Gauicol was the substance we used as a reducing
Claire Bell
Biology 1130
Jazmen Myers
Section 36
Abstract Peroxidase is a turnip enzyme; it is used in the oxidation of hydrogen peroxide to lower activation energy, speeding up the reaction. The activity of peroxidase is highly dependent on its environment and most importantly the pH level. Peroxidase has been the focus of many recent studies and is believed to possibly reduce swelling among other things. We conducted an experiment testing the effect different levels of pH had on the reaction rate of peroxidase. In the experiment we created different solutions all containing hydrogen peroxide, peroxidase, and guaiacol. However each cuvette contained a different pH level, 2,5,7,or 10. Through the experiment we found the activity of peroxidase is highest at its optimal pH level between 6.0 and 7.0 and ceases to react at pH levels 2.5 and lower and between 8.5 and 9.5 and higher. Our data supported the idea that peroxidase is slightly acidic enzyme; however the more acidic pH levels are more effective as inhibitors opposed to the highly basic pH levels.
Introduction
In this experiment we tested the effects of the environmental parameter pH on peroxidase activity. We performed this experiment to observe and analyze the effect pH has on peroxidase activity and find the optimal pH level for peroxidase, as well as the ceasing point in the reaction. Parameters are characteristics that can alter an experiment. The parameter we used in this experiment was pH. The pH scale ranges from levels 0 to 14, 0 being the most acidic, and 14 being the most basic. An enzyme is a biological catalyst made up of proteins. Enzymes speed up reactions by bringing reactants close together and lowering the activation energy. Enzymes are extremely specific to their substrates. A substrate is material where an organism lives or the surface upon which an organism grows or is attached. Gauicol was the substance we used as a reducing