After recognizing that the first and second generation of biodiesels is from sugar, vegetable oils and lignocellulosic biomass, microalgae are now known as the ‘third generation of biodiesel’. As many studies have told us that microalga is a promising organism for mass production of biomass and biofuel which are renewable and environmental friendly. Microalgae have a higher growth rate and productivity as compared to plants. Most microalga species are easily adaptable to climate changes and are not limited by seasonal culture, which also says that products of microalgae can be harvested daily. Microalgae can store many valuable products such as lipids and carbohydrate due to the high efficiency of carbon fixation. (Shih-Hsin Ho, 2012)1 These favorable characteristics of microalgae result in a more effective way to generate a sustainable biomass source.
By further enhancing the efficiency of carbon dioxide fixation of microalgae, it will also increase the productivity. Many factors are to be considered to improve the rate of carbon fixation effectively. The factors are categories into two sectors, physical and chemical conditions. Under the physical conditions, factors include: depth of culture, type of culturing, light intensity and cell density. Chemical conditions include: species and strains of microalgae, purity of culture, nutrients assimilation and carbon supply.
Chemical conditions
Selection of the strain of microalgae is vital in the enhancement of biomass, biochemical and biofuel production. This is because different species and strains have different rate of cell activity. For instance, Scenedesmus obliquus CNW-N is a better strain to fix carbon and produce biomass compared to other strain such as S. obliquus ESP-7. (Shih-Hsin Ho, 2012)1
Cell cultures are best when it is free from any contamination. Contaminations can