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Ammonium Persulfate Vs TEMED

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Ammonium Persulfate Vs TEMED
Many reagents were used in this experiment. For the stacking gel and resolving gel, the APS and TEMED were added at the end because they polymerize the gel immediately. Ammonium Persulfate (APS) is an oxidizing substance that is used along TEMED to catalyze the polymerization of acrylamide. It instructs polymerization. TEMED hurries the formation rate of the free radicals from persulfate and these in turn catalyze polymerization. The persulfate free radicals switch acrylamide monomers to free radicals, which react with incapacitated monomers to arise the polymerization chain reaction. Both APS and TEMED are used to make the reaction faster.
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