Atividade do Extrato de Própolis-Etanólico em Pacientes com Implantes Orais para diminuição de bactérias

The 2nd Kurdistan Conference on Biological Sciences

J. Duhok Univ. Vol.12, No.1 (Special Issue), Pp 233-239, 2009

University of Duhok 6-8 May, 2008

ANTIMICROBIAL ACTIVITY OF PROPOLIS COLLECTED IN DIFFERENT
REGIONS OF SULAIMANI PROVINCE-KURDISTAN REGION/IRAQ
HASTYAR H. NAJMADEEN and FARHAD A. KH. K AKAMAND
Dept of Biology, College of Science, University of Sulaimani, Kurdistan region, Iraq
(Accepted for publication: November 19, 2008)

ABSTRACT
The aim of this study was to investigate the activity of (28%) ethanolic extract propolis (EEP) of [Bazian, Pshdar, Sharbazher,
Khormal, Kanakawa and Sulaimani (city center)] against (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli,
Pseudomonas aerugenosa, Klebsiella pneumoniae, Proteus mirabilis and yeast Candida albicans). Two methods were employed for propolis activity evaluation; firstly by agar well diffusion method using 40µl of propolis extracts and ethanol 70% as the solvent control per well against tested bacteria and fungus and more commonly used antibiotics were used in comparison to propolis samples. The second method was employed by measuring Minimal Inhibitory Concentration & Minimal Bactericidal; Fungicidal
Concentrations (MIC, MBC & MFC) using two fold dilution method for propolis extracts and ethanol 70% as a solvent control in serial concentrations. The Results exhibited that propolis is more efficient than some antibiotic discs. The most sensitive bacteria against (EEP) with mean inhibitory diameters (22-26) mm was S. epidermidis followed by S. aerues and C. albicans (15-22) mm.
Tested Gram negative bacteria have lowest inhibitory zones (7-9) mm. Results of MIC & MBC showed S. aureus with (0.1750.7mg/ml) followed by S. epidermidis & C. albicans with (0.7-1.4mg/ml). Among tested Gram negative bacteria, all (EEP) samples showed relatively strong inhibitory action on P. mirabilis (14mg/ml) and weak inhibitory action on P. aerugenosa & K. pneumoniae
(28mg/ml) of

References: - Abd El Hady; F. K. and Hegazi,;A. G. (2002). Egyptian Proplois: 2 - Bankova, V. S. ; De Castro, S. L. and Marcucci, M. C. (2000). - Hegazi,A.G. and Abd El Hady, F. K. (2002). Egyptian Proplois: 3 - Hegazi, A. G. (2004). Antifungal and antibacterial activity of Egyptian Propolis - Kosalec, I.; Pepeljnjak,S.;Bakmaz,M., and Vladimir-Knezevic, S.(2005).Flavonoid analysis and antimicrobial activity of - Gebara, E. C. E.; Lima, L. A. and Mayer, M. P.A. (2002). - Oksuz, H.; Duran, N.; Tamer, C.; Cetin, M.; and Silici, S. (2005). University of Duhok 6-8 May, 2008 Staphyloccucus aureus Keratitis in Rabbits - Katircioglu, H. and Mercan, N. (2006). Antimicrobial activity and chemical compositions of Turkish propolis form different - Teixeira, E. W.;Message,D.; Negri,G.; and Salatino, A. (2006). - Ansorge, S.; Reinhold, D.; and Lendeckl, U. (2003) Propolis and some of its constituents down-regulate DNA synthesis and - Bankova, V. (2000). Determining quality in propolis samples. - Lisowski, F.(1984). Demystifying health food.Contin. 1- Burdock, G. A. (1998). Review of the biological properties and toxicity of bee propolis (2001).The antibacterial activity of propolis produced by Apis mellifera L.and Brazilian stingless bees,J.Venom.Anim. - Bankova, V.; De Castro, S. and Marcucci, M. (2000). Propolis: recent advances in chemistry and plant origin - Korel, W.; Scheller, S.; Shani, J.; Pietsz, G. and Czuba, Z. (1993). - Mirzoeva, O.; Grishanin, R. and Calder, P. (1997). Antibacterial action of propolis and some of its components: the effects of - Tajaisi-Kikuni, N. and Schilcher, H. (1994). Electron microscopic and microcalorimetric investigations of the possible