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Baking Soda Lab Report

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Baking Soda Lab Report
Materials/Equipment:
• Plastic syringes with Luer-Lock, 30 mL
• Space Sand
• Scissors.
• Cotton rounds
• Clear plastic cups or drinking glasses
• Permanent marker & Safety goggles.
• Isopropyl alcohol
• Distilled water
• Grape soda
• Lab notebook.
Procedures:
1. In the lab notebook, you would have to make data tables like Table 1 and Table 2. You would have to put down data
2. Get out three plastic cups or drinking glasses. Pour some 70% isopropyl alcohol into one, distilled water into another, and grape soda into the last cup.
3. Get out six more plastic cups and label them Waste, 1st Fraction, 2nd Fraction, 3rd Fraction, 4th Fraction, and 5th Fraction. It will be easiest to arrange them in a line in front of you, going left to right.
4. Remove one
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First equilibrate your column with the mobile phase and some distilled water. This will prepare it for processing the grape soda. Once all the alcohol has dripped out, slowly remove the plunger out of syringe A, but continue to hold it over the waste cup. Tilt the plunger back and forth so as it does not "pop" when you pull it out.
8. Now add a sample of grape soda to your equilibrated column.
9. Next, add 5 mL of distilled water to the column for better color separation.
10. Next, add your 70% isopropyl alcohol mobile phase to elute the colors from the column.
11. Lastly, add some more of your 70% isopropyl alcohol to the column. data tables in your lab notebook.
12. If you want to, you can take pictures of your eluates in the different cups. You should include a sample of the original grape soda in a cup for comparison. You may want to take pictures using a white background, such as a sheet of blank paper, and include labels. You could include these pictures on your
13. Repeat steps 4–11 two more times. Be sure to record your observations in the data tables in your lab notebooks, in the rows for trials 2 and 3.
a. For syringe "A," use a new syringe, but you can use the same syringe as before for syringe

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