Workshop 2
Bacteria
Purpose of Bergy’s Manual: based on ribosomal RNA sequences, which presumably reflect phylogenetic (evolutionary) relationships. Used for the identification of prokaryotes. 2nd edition on classification of prokaryotes.
4 Tests/ Parameters that are evaluated to classify bacteria are: procedure’s that determine an organism’s ability to ferment carious carbohydrates; utilize various substrates such as specific amino acids, starch, citrate, and gelatin; or produce waste products such as hydrogen sulfide gas and also differences in fatty acid composition of bacteria are also used to distinguish between bacteria.
4 Divisions of procaryotic bacteria per Bergy’s Manual are: Gracillicutes, Firmicutes, Tenericutes, and Mendosciutes
What kingdoms are procaryotic organisms classified? Monera and
What domains are procaryotic organisms classified? Archae and Bacteria
Description of the following including, Gram stain, Morphology, and Genus species of common representative organisms & significant characteristics organisms & examples
Enterobacteriaceae (enterics) -Gram neg., rod shaped fermenting sugars to produce lactic acid. Ex: Salmonella, E. coli, Shigella
Micrococcaceae- Gram pos., cocci (round),
Streptococcaceae- Gram pos., Ex: Streptococcus, Lactococcus, Lactovum
Mycoplasmataceae- Gram neg., nonmotile microorganism,
Mycobacteriaceae- Gram pos., aerobic and nonmotile, genus of Actinobacteria. Ex: Tuberculosis
Rickettsiaceae- Gram neg., sensitive to environmental exposure. Genus-Rickettsia. Ex: Ticks, Lice
Chlamydiaceae- Gram neg., Phylum Chlamydiae, Ex: Chlamydia, C. trachoma is, C. suis
7. A 27 year old white female presented at the walking clinic of her local physic al on August 15. On physical exam, the patient had a fever of 38.5C. She appeared fatigued, had tender joints, and complained of a headache, a stiff neck and a backache. The physician noticed a circular “rash” about 5 inches in diameter, with a bright red