Biology
Protein
BIOL160: Lab Report 1
January 30, 2013
Lab partners: Park Jin Mun 0304299 Tan Zhi Chen 0306521
Hypothesis
1) If a calculated absorbance level is directly related to the concentration of albumin, then a higher concentration of albumin will lead to higher absorbance level.
Results
Table No. | Albumin(cm3) | KCl (mL) | Biuret Reagent (mL) | Absorbance | 1 | 0 | 5 | 3 | 0.049 | 2 | 1 | 4 | 3 | 0.112 | 3 | 2 | 3 | 3 | 0.258 | 4 | 4 | 1 | 3 | 0.39 | 5 | 5 | 0 | 3 | 0.418 | 6 | 5 (A) | 0 | 3 | 0.198 | 7 | 5 (B) | 0 | 3 | 0.347 |
Table 1) Test Result
Table 1 is the results of seven test tubes with a different amount of solutions. 3 mL of Biuret Reagent was added to all seven test tubes and absorbance was measured with spectrophotometer. It is observed that the absorbance level increases as the amount of albumin is added more in different test tubes, while the amount of KCl decreases.
Concentration of albumin in mg(x-axis) | Absorbance (y-axis) | 0 | 0.049 | 2.5 | 0.112 | 5 | 0.258 | 10 | 0.39 | 12.5 | 0.418 | (A) | 0.198 | (B) | 0.347 |
Table 2) Analyze of two factors, concentration of albumin and absorbance The concentration of albumin in the standard albumin is 2.5 mg/cm3of 0.5 M KCl. Hence 1.0 cm3of albumin is calculated to 2.5 mg of the concentration of albumin. For 6th and 7th test tube were unknown labeled as (A) and (B).
Y= a+ bx
( Where b= nxiyi- xi yinxi2- (xi )2 , a =yin-bxin)
Figure 1) Statistical formula for the equation of the graph This equation is to calculate the exact gradient of Table 2. By using the equation, the unknown
BIOL160: Lab Report 1
January 30, 2013
Lab partners: Park Jin Mun 0304299 Tan Zhi Chen 0306521
Hypothesis
1) If a calculated absorbance level is directly related to the concentration of albumin, then a higher concentration of albumin will lead to higher absorbance level.
Results
Table No. | Albumin(cm3) | KCl (mL) | Biuret Reagent (mL) | Absorbance | 1 | 0 | 5 | 3 | 0.049 | 2 | 1 | 4 | 3 | 0.112 | 3 | 2 | 3 | 3 | 0.258 | 4 | 4 | 1 | 3 | 0.39 | 5 | 5 | 0 | 3 | 0.418 | 6 | 5 (A) | 0 | 3 | 0.198 | 7 | 5 (B) | 0 | 3 | 0.347 |
Table 1) Test Result
Table 1 is the results of seven test tubes with a different amount of solutions. 3 mL of Biuret Reagent was added to all seven test tubes and absorbance was measured with spectrophotometer. It is observed that the absorbance level increases as the amount of albumin is added more in different test tubes, while the amount of KCl decreases.
Concentration of albumin in mg(x-axis) | Absorbance (y-axis) | 0 | 0.049 | 2.5 | 0.112 | 5 | 0.258 | 10 | 0.39 | 12.5 | 0.418 | (A) | 0.198 | (B) | 0.347 |
Table 2) Analyze of two factors, concentration of albumin and absorbance The concentration of albumin in the standard albumin is 2.5 mg/cm3of 0.5 M KCl. Hence 1.0 cm3of albumin is calculated to 2.5 mg of the concentration of albumin. For 6th and 7th test tube were unknown labeled as (A) and (B).
Y= a+ bx
( Where b= nxiyi- xi yinxi2- (xi )2 , a =yin-bxin)
Figure 1) Statistical formula for the equation of the graph This equation is to calculate the exact gradient of Table 2. By using the equation, the unknown
References: Department of Diary and Food Industry, B.C Johnson and M.Swabsib, 2008, University of Wisconsin, Modison. Hillsborough Community College, Ybor City Campus Piedmont Community College, < http://www2.piedmontcc.edu/> Refer from Wiki.answer.com <http://wiki.answers.com/Q/How_does_biuret 's_reagent_work_in_protein_testing>.