Blood Glucose Regulation
LABORATORY REPORT
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Activity:
Name:
Instructor:
Date:
Blood Glucose Regulation
Sandra Shakur
January 25, 2015
Predictions
1. Plasma glucose levels will be highest immediately after the meal (0 hr).
2. Plasma ketone levels will be highest
1-3 hours after the meal.
3. Plasma insulin levels will be highest before the meal (fasting).
4. Plasma glucagon levels will be highest before the meal (fasting).
Materials and Methods
1. Name the Dependent Variable. plasma levels of glucose, ketones, insulin, and glucagon
2. Name the Independent Variable. food and beverage intake
3. Name the Controlled Variables. gender, age, BMI; physical activity, caffeine and alcohol intake
4. Why were physical activity and caffeine and alcohol intake the controlled variables?
Caffeine and alcohol intake can be controlled same as the amount of physical activity.
5. What type of assay was used to measure plasma glucose and ketone levels?
Glucose, Oxidase, Peroxide, and Dianisdine.
6. What type of assay was used to measure plasma insulin and glucagon levels?
Glucose, Oxidase, Peroxide, and Dianisdine.
Results
Table 2: Glucose and Ketone Concentration in Blood Plasma
Fasting
Immediately post meal
I hr post meal 2 hr post meal 3 hr post meal Sub. 1
5.3
6.4
Glucose (mmol/l)
Sub. 2
Sub. 3
5.2
5.2
6.4
6.4
average
5.2
6.4
Sub. 1
123
110
Ketones (µmol/l)
Sub. 2
Sub. 3
110
101
90
106
average
111
102
6.2
6.3
6.4
6.3
73
58
62
64
5.6
5.6
5.6
5.6
72
76
51
66
4.7
4.6
4.6
4.6
54
62
64
60
Glucose Concentration in Blood Plasma
Ketone Concentration in Blood Plasma
1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest.
Glucagon concentration was the highest 2 hours post meal
2. When was plasma glucose concentration lowest? If values are similar for several
(Click on the Save a Copy button on the panel above to save your report)
Activity:
Name:
Instructor:
Date:
Blood Glucose Regulation
Sandra Shakur
January 25, 2015
Predictions
1. Plasma glucose levels will be highest immediately after the meal (0 hr).
2. Plasma ketone levels will be highest
1-3 hours after the meal.
3. Plasma insulin levels will be highest before the meal (fasting).
4. Plasma glucagon levels will be highest before the meal (fasting).
Materials and Methods
1. Name the Dependent Variable. plasma levels of glucose, ketones, insulin, and glucagon
2. Name the Independent Variable. food and beverage intake
3. Name the Controlled Variables. gender, age, BMI; physical activity, caffeine and alcohol intake
4. Why were physical activity and caffeine and alcohol intake the controlled variables?
Caffeine and alcohol intake can be controlled same as the amount of physical activity.
5. What type of assay was used to measure plasma glucose and ketone levels?
Glucose, Oxidase, Peroxide, and Dianisdine.
6. What type of assay was used to measure plasma insulin and glucagon levels?
Glucose, Oxidase, Peroxide, and Dianisdine.
Results
Table 2: Glucose and Ketone Concentration in Blood Plasma
Fasting
Immediately post meal
I hr post meal 2 hr post meal 3 hr post meal Sub. 1
5.3
6.4
Glucose (mmol/l)
Sub. 2
Sub. 3
5.2
5.2
6.4
6.4
average
5.2
6.4
Sub. 1
123
110
Ketones (µmol/l)
Sub. 2
Sub. 3
110
101
90
106
average
111
102
6.2
6.3
6.4
6.3
73
58
62
64
5.6
5.6
5.6
5.6
72
76
51
66
4.7
4.6
4.6
4.6
54
62
64
60
Glucose Concentration in Blood Plasma
Ketone Concentration in Blood Plasma
1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest.
Glucagon concentration was the highest 2 hours post meal
2. When was plasma glucose concentration lowest? If values are similar for several