Brine Shrimp and Ethanol Alcohol
The Effect of Ethanol on the Viability of Artemia salina as Environmental Indicators
Biology 151 Laboratory
ABSTRACT Artemia salina are used as environmental indicator species. They are used to test changes in their environment. A. salina is sensitive to toxins, temperature, and drugs. For the experiment, cysts were put into different amounts of ethanol alcohol and their viability was measured. The hypothesis used was if there is more ethanol alcohol, then the viability of the brine shrimp is unfavorable. The brine shrimp were put into sixteen Petri dishes with the same amount of brine solution. Different amounts of ethanol alcohol were added to each Petri dish. After 168 hours, the brine shrimp were looked at under a microscope and some cysts became nauplii and many died. In the control dishes, with no alcohol, the viability was the lowest; the average viability for treatment one was not the lowest but treatment four was the lowest. Treatment two had the highest viability (Table 1). Treatment one, 0.1668, versus treatment three, 0.1224, and treatment four, 0.0478, confirms that small amounts of ethanol alcohol can be accepted by the A. salina. The numbers of cysts in each Petri dish were not consistent. If the number of cysts were consistent then the comparison between viabilities in each dish would be more accurate. The importance of the average viability is that it is used as representative data. It will help show the inaccuracies of the data recorded. The purpose of this experiment is determine the aptness of A. salina as warnings of ethanol pollution.
INTRODUCTION
Artemia, or commonly known as brine shrimp, live in hyper-saline lakes. Artemia begin as dormant cysts, which contains an embryo in a diapause state. When water temperature and salinity changes occur, the cysts rehydrate and releases the first growth stage, known as nauplius larva. The cysts are very small and about fifty could fit
Biology 151 Laboratory
ABSTRACT Artemia salina are used as environmental indicator species. They are used to test changes in their environment. A. salina is sensitive to toxins, temperature, and drugs. For the experiment, cysts were put into different amounts of ethanol alcohol and their viability was measured. The hypothesis used was if there is more ethanol alcohol, then the viability of the brine shrimp is unfavorable. The brine shrimp were put into sixteen Petri dishes with the same amount of brine solution. Different amounts of ethanol alcohol were added to each Petri dish. After 168 hours, the brine shrimp were looked at under a microscope and some cysts became nauplii and many died. In the control dishes, with no alcohol, the viability was the lowest; the average viability for treatment one was not the lowest but treatment four was the lowest. Treatment two had the highest viability (Table 1). Treatment one, 0.1668, versus treatment three, 0.1224, and treatment four, 0.0478, confirms that small amounts of ethanol alcohol can be accepted by the A. salina. The numbers of cysts in each Petri dish were not consistent. If the number of cysts were consistent then the comparison between viabilities in each dish would be more accurate. The importance of the average viability is that it is used as representative data. It will help show the inaccuracies of the data recorded. The purpose of this experiment is determine the aptness of A. salina as warnings of ethanol pollution.
INTRODUCTION
Artemia, or commonly known as brine shrimp, live in hyper-saline lakes. Artemia begin as dormant cysts, which contains an embryo in a diapause state. When water temperature and salinity changes occur, the cysts rehydrate and releases the first growth stage, known as nauplius larva. The cysts are very small and about fifty could fit
Cited: "Brine Shrimp and Ecology of Great Salt Lake." USGS Utah Water Science Center: Great Salt Lake. U.S. Geological Survey, 10 Jan. 2013. Web. 04 Apr. 2013. . Gajardo, Gonzalo M., Patrick Sorgeloos, and John A. Beardmore. "Abstract." National Center for Biotechnology Information. U.S. National Library of Medicine, 28 Nov. 2006. Web. 04 Apr. 2013. . Lu, Yin, Xiaolu Xu, Tian Li, Yifei Xu, and Xu Wu. "The Use of a Brine Shrimp (Artemia Salina) Bioassay to Assess the Water Quality in Hangzhou Section of Beijing-Hangzhou Grand Canal - Springer." The Use of a Brine Shrimp (Artemia Salina) Bioassay to Assess the Water Quality in Hangzhou Section of Beijing-Hangzhou Grand Canal - Springer. N.p., 01 Mar. 2012. Web. 04 Apr. 2013. .