Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A, B, C, and D. In activity A, the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction, to study the characteristics of an enzyme mediated reaction, and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H2O2 present in a solution. The amount will be the baseline for activities C and D. The purpose of activity C is to determine the rate at which H2O2 spontaneously decomposes when exposed to room temperatures and ambient light for 24 hours. The purpose of activity D is to determine the rate at which catalase decomposes H2O2. After adding H2SO4 for different time lashes, etc., the resulting data will be graphed at which the catalase decomposed by catalase.…
Enzymes are biological catalysts or assistants that consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. They can either launch a reaction or speed it up. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). The catalase used in this experiment will come from five different sources: Spinacia oleracea (Spinach), Brassica oleracea (Broccoli), Solanum tuberosum (Russet Potato), Malus domestica (Apple), and Allium cepa (Onion). The five different catalases from the sources will all be used to catalyze Hydrogen peroxide (H2O2). When hydrogen…
The Effect of Different Concentrations of the Enzyme Catechol Oxidase on the Rate of Benzoquinone Production When Mixed with Pure Catechol…
We found a study about the effects of glycosylation and pH conditions in the dynamics of human arylsuffatase A. They concluded that lowering pH and increasing glycosylation reduced the flexibility of the enzyme. Also, at acidic pH levels the glycosylated enzyme had a higher conformational stability. This study was similar to ours in that it investigated how pH conditions affect enzyme functionality. They found optimal pH conditions for the enzyme like we did, but they took into account another variable that is that influenced functionality…
6. Plug in the Go Link onto the USB drive of the computer (See Figure 1)…
Catechol oxidase is found in animals and plants. Catechol oxidase is the enzyme that catalyzes the production of Ortho- quinone. This reaction produces Ortho- quinone, water, and browning of a potato when exposed to air. The purpose of this experiment was to observe the behavior of reaction rate when enzyme or substrate concentration is changed.…
Brinkman, FG & Sminia, T. 1977. Histochemical location of catalase in Peroxisomes and of Peroxidase in Cell walls and Golgi Bodies of Cells in Differentiating Potato Tuber Tissue. Zeitschrift für Pflanzenphysiologie. Accessed 10/12/15. Available from: http://www.sciencedirect.com/science/article/pii/S0044328X77802316…
Prediction: As the temperature increases the rate of enzyme activity will also increase, thus increasing the rate of reaction. However, if the temperature is too high the enzyme will denature.…
For the room temperature experiment the water had a starting temperature of 24 degrees Celsius and a pH of 6. The vinegar had a starting pH of 1 and a temperature of 24 degrees Celsius. During the time the whole and crushed chalk were in the water nothing happened besides the water turning a little bit cloudy. While in the vinegar the whole chalk broke down, turned the water a tint of yellow, and produced foam of 1 millimeter. The crushed chalk turned the water a cloudy white and produced foam of 12…
Enzymes are created from proteins and catalyze reactions. “Catechol oxidase if found widely in both plants and animals [that] catalyzes the oxidation of catechol to ortho-quinone and water” (Schultz 6). For this experiment, each group utilized a spectrophotometer which measures the absorbance of the mixture. The outcomes of part one displayed that our average reaction rate with the substrate was 0.070739091, while our average reaction rate without the substrate was 0.008745455. The results of part two revealed that our average absorbance at the different enzyme concentration on reaction rate for the full-strength serial dilution was 0.0795, the ½ concentration was 0.0666667, the ¼ concentration was 0.0616667, and the 1/8 concentration was 0.045.…
The purpose of this lab was to test if ethanol affects the reaction involving hydrogen peroxide and catalase. Tests were performed by putting chicken liver, ethanol solution (diluted ethanol solution for other trials) and hydrogen peroxide in a test tube with a side arm, and having a rubber tube lead the oxygen gas into a gas collection tube. Results from the tests showed a negative correlation, this means that the more diluted the solution of 95% ethanol was, the less oxygen gas collected. Controlling a number of factors which include human error, temperature change, pH levels, substrate concentrations and ensuring a controlled experimental environment will work to increase the accuracy of the experiment.…
In this laboratory exercise we studied enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment.…
Research Question: How will an increase of FeSO4 concentration affect the activity of the enzyme catalase? Hypothesis: By increasing the concentration of FeSO4, there will be a decrease in the activity of the enzyme catalase because the Iron (ii) in the metallic ion will act as an inhibitor during the catalysis of the hydrogen peroxide into O2 and H2O. Introduction: We rely on oxygen to provide us with energy (ATP) and go through all the phases of aerobic respiration. However, oxygen can be harmful to humans and can cause problems if not carefully regulated.…
➢ Temperature of water bath at 25 oC : Differing temperature affects the rate of activity of an enzyme. ➢ Test tube: Using the same type of test tube for all the trials. ➢ Time interval: The amount / length of foam formed in 45 seconds using a stop watch recorded in cm Materials Required: 1.…
In order to determine the acidity of certain substances, we used moist and dry pH paper (litmus tests), and observed the results. First we used dry pH paper to test the acidity of household ammonia, and it turned yellow. According to the legend, this indicates a weak acid. This is, however, not accurate as the vapor of the household ammonia wasn’t able to react as strongly with the pH paper since it was dry.…