Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A, B, C, and D. In activity A, the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction, to study the characteristics of an enzyme mediated reaction, and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H2O2 present in a solution. The amount will be the baseline for activities C and D. The purpose of activity C is to determine the rate at which H2O2 spontaneously decomposes when exposed to room temperatures and ambient light for 24 hours. The purpose of activity D is to determine the rate at which catalase decomposes H2O2. After adding H2SO4 for different time lashes, etc., the resulting data will be graphed at which the catalase decomposed by catalase.…
In this lab the peroxidase enzyme is tested in a dormant avocado seed as well as an avocado seed undergoing the process of germination. A gas pressure will be used to test the seeds and see if the peroxidase enzyme is present in either of the seeds.…
Enzymes are biological catalysts or assistants that consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. They can either launch a reaction or speed it up. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). The catalase used in this experiment will come from five different sources: Spinacia oleracea (Spinach), Brassica oleracea (Broccoli), Solanum tuberosum (Russet Potato), Malus domestica (Apple), and Allium cepa (Onion). The five different catalases from the sources will all be used to catalyze Hydrogen peroxide (H2O2). When hydrogen…
The Effect of Different Concentrations of the Enzyme Catechol Oxidase on the Rate of Benzoquinone Production When Mixed with Pure Catechol…
We found a study about the effects of glycosylation and pH conditions in the dynamics of human arylsuffatase A. They concluded that lowering pH and increasing glycosylation reduced the flexibility of the enzyme. Also, at acidic pH levels the glycosylated enzyme had a higher conformational stability. This study was similar to ours in that it investigated how pH conditions affect enzyme functionality. They found optimal pH conditions for the enzyme like we did, but they took into account another variable that is that influenced functionality…
This lab was performed in order to discover the activity of the enzyme catecholase in different pH levels as well as its absorbance in differently concentrated solutions. A spetrophotometer was used to measure the absorbance of the enzyme catecholase in different pH solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close to neutral. When pH was acidic or basic, the catecholase was less effective. Also, when there was a higher concentration of potato juice and a lower concentration of phosphate buffer, absorbance of the enzyme increased.…
Catechol oxidase is found in animals and plants. Catechol oxidase is the enzyme that catalyzes the production of Ortho- quinone. This reaction produces Ortho- quinone, water, and browning of a potato when exposed to air. The purpose of this experiment was to observe the behavior of reaction rate when enzyme or substrate concentration is changed.…
Brinkman, FG & Sminia, T. 1977. Histochemical location of catalase in Peroxisomes and of Peroxidase in Cell walls and Golgi Bodies of Cells in Differentiating Potato Tuber Tissue. Zeitschrift für Pflanzenphysiologie. Accessed 10/12/15. Available from: http://www.sciencedirect.com/science/article/pii/S0044328X77802316…
Enzyme Kinetics and Protein Determination: How Enzyme Catalase Concentration Affects Reaction Rate and Determining the Identity of Unknown Proteins through Absorbance…
For Activity A, we first tested enzyme activity. First, we used an H2O2 syringe to transfer 10 mL of H2O2 into an unlabeled 60-mL cup. Then, we used a transfer pipet to add one mL of catalase solution into the unlabeled 60-mL cup that we put H2O2 in. After that, we observed the solution for one minute. Then we tested the effect of boiling on enzyme activity. First we used a transfer pipet to transfer 4 mL of catalase into a test tube. After that, we placed the test tube filled with catalase in a boiling water bath for five minutes. While we were waiting, we rinsed the unlabeled cup we used earlier when we tested enzyme activity. Then we used a H2O2 syringe to transfer 10 mL of H2O2 into the rinsed unlabeled cup. After five minutes, we transferred 1 mL of the boiling catalase into the unlabeled cup with H2O2 in it with an unused transfer pipet and observed the results. After testing the effect of boiling on enzyme activity, we tested for catalase in living tissue. First, we rinsed the unlabeled 60 mL cup we used earlier. Then, we used a scalpel to cut a small piece of liver. After that, we macerated the piece of liver with a glass rod. When the liver was macerated enough, we put it in a cup with 10 mL of H2O2, which was transferred into the cup with a H2O2 syringe. Lastly, we observed the cup.…
1) The purpose of this lab was to determine the rate of enzyme activity under variety of different conditions, such as, different amount of drops of enzymes and different temperature of water. The class measured the pressure in the test tube during the reaction of the substance with, 1.5 ml of H2O2, 1.5ml of H2O and different amounts of enzyme drops, to determine how much oxygen gas is produced during the reaction since the pressure of the test tube will get higher as more oxygen gas is accumulated during the reaction.…
a cleft or depression. Substrates will bind to the active site of an enzyme if it is the…
In this laboratory exercise we studied enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment.…
Introduction: This lab tested how enzymes are able to affect the rate of chemical reactions and how the rate of which an enzyme works in different conditions. The conditions the enzymes were tested in included…
This experiment is to study and measure the enzyme activity using a spectrophotometer and to understand the practical aspects of handling enzymse. This is done when the colourless ONPG is split into galactose and o-Nitrophenol (a yellow compound). The higher the enzyme concentration, the higher the absorbance value. Different molecules absorb different wavelengths of light. For this experiment, the spectrophotometer is set at 420nm, so that we can obtain the best absorbance results.…