Chainobead lab

Part 1A Analysis questions:
1. How many “chainobeads” was your enzyme able to make per minute in the 0 – 15 second interval?
Our enzyme was able to make 6 chainobeads in the 0-15 interval.

2. How many “chainobeads” was your enzyme able to make per minute in the 60 – 120 second interval?
Our enzyme was able to make 49 chainobeads in the 60-120 intervals.

3. Did your enzyme's rate change over time? How does this compare to a real enzyme?
The enzyme’s rate did change over time. This compares to a real enzyme because an enzyme’s job is to speed up the reactions and as time allotted. That did happen since the enzyme in our lab was able to make more chainobeads as time progressed.
4. Graph

5. Table
Chainobead Construction Time Part A
15 Seconds
6
30 Seconds
12
60 Seconds
20
120 Seconds
29

Part 1B:
1. The results of the 120 seconds with the non-pop beads added to the mix didn’t hinder the enzyme’s construction rate at all except for the first trial. In the first trial 28 chainobeads were constructed, in the second trial 30 chainobeads were constructed, and in the final trial 35 chainobeads were constructed. The results may have varied since our enzyme became very good at handling and constructing the chainobeads.
2. Graph

3. Table
Chainobead Construction with Non-Pop beads
120 Seconds
28
120 Seconds
30
120 Seconds
35

Part 1C:
1. C. What happens to the reaction rate if the enzyme wears a pair of latex gloves?
If the enzyme were to wear a pair of latex gloves the reaction rate would slow down because the pop-beads will most likely stick to the latex coding making it a tad bothersome for the enzyme to construct the chainobeads
2. Graph

3. Table
Chainobead Construction with a Latex Glove Part C
120 Seconds
26

Additional analysis questions for Part 1:
1. Which part of this model represents the Chainobead polymerase enzyme?
The person constructing the chainobeads represents the chainobead polymerase enzyme.

2. Which part of this model represents the substrate?