Conditions Needed for Effective Enzyme Action
Jolly Nguyen 11C
Conditions needed for effective enzyme action
Aim: To investigate the activity of enzymes and how might the activity be effected in different conditions.
Hypothesis:
Temperature rises and change of substrate concentration may cause denaturation of the protein of enzymes. So as the temperature rises the amount of active enzyme progressively decreases, and the rate is slowed. Exposure to heat causes atoms to vibrate violently and this disrupts bonds within globular proteins.(Clegg, 2007). Moreover, at lower concentration, the rate increases in direct proportion to the substrate concentration. (Clegg, 2007)
Materials: Fresh liver (will be cut into 12 small cubes with equal size). Frozen works fine | Thin spatulas to scrape liver from mortar into test tube | 4 test tubes and stand | Fine sand | 3% hydrogen peroxide | 100mL breaker | Mortar and pestle | 10 mL Measuring cylinder | Bunsen burner/hot plate | Detergent | 1M Hydrochloric acid (HCL) | Ruler | Matches | Knife |
Procedure: 1. Cut 12 small cubes of liver (under 1 cm), of equal size. 2. Put 3 liver cubes into a test tube half filled with water and place into a beaker of boiling water for 5 minutes. 3. Place 3 liver cubes into the mortar with a small quantity if sand then grind with the pestle to form a paste. 4. Label 4 test tubes A,B,C and D and put 5ml of 3% hydrogen peroxide into each tube, followed by 3 drops of detergent in each tube. 5. Place 3 fresh liver cubes into test tube A. observe the reaction. 6. Measure the height of the bubbles formed and record the result in table1. Record any qualitative observations in Table2. 7. Place the ground liver into test tube B and record as before. 8. Place the boiled liver cubes into test tube C and record results in both tables. 9. In tube D, add 5 drops of 1M HCl to the test tube, then add the final 3 fresh liver cubes into the test tube and record results in both tables.
(Year10
Conditions needed for effective enzyme action
Aim: To investigate the activity of enzymes and how might the activity be effected in different conditions.
Hypothesis:
Temperature rises and change of substrate concentration may cause denaturation of the protein of enzymes. So as the temperature rises the amount of active enzyme progressively decreases, and the rate is slowed. Exposure to heat causes atoms to vibrate violently and this disrupts bonds within globular proteins.(Clegg, 2007). Moreover, at lower concentration, the rate increases in direct proportion to the substrate concentration. (Clegg, 2007)
Materials: Fresh liver (will be cut into 12 small cubes with equal size). Frozen works fine | Thin spatulas to scrape liver from mortar into test tube | 4 test tubes and stand | Fine sand | 3% hydrogen peroxide | 100mL breaker | Mortar and pestle | 10 mL Measuring cylinder | Bunsen burner/hot plate | Detergent | 1M Hydrochloric acid (HCL) | Ruler | Matches | Knife |
Procedure: 1. Cut 12 small cubes of liver (under 1 cm), of equal size. 2. Put 3 liver cubes into a test tube half filled with water and place into a beaker of boiling water for 5 minutes. 3. Place 3 liver cubes into the mortar with a small quantity if sand then grind with the pestle to form a paste. 4. Label 4 test tubes A,B,C and D and put 5ml of 3% hydrogen peroxide into each tube, followed by 3 drops of detergent in each tube. 5. Place 3 fresh liver cubes into test tube A. observe the reaction. 6. Measure the height of the bubbles formed and record the result in table1. Record any qualitative observations in Table2. 7. Place the ground liver into test tube B and record as before. 8. Place the boiled liver cubes into test tube C and record results in both tables. 9. In tube D, add 5 drops of 1M HCl to the test tube, then add the final 3 fresh liver cubes into the test tube and record results in both tables.
(Year10
Bibliography: Clegg, C. 2007. Biology for the IB DIPLOMA, Hodder Murray, London, p.54,56,60.