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Determination of Iron in Natural Water by Spectrophotometry.

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Determination of Iron in Natural Water by Spectrophotometry.
Title: Determination of Iron in Natural water by Spectrophotometry.
Aim: To determine the iron in natural water by spectrophotometry.
Abstract: The iron in natural water was determined by utilizing spectrophotometric analysis. That was done by measuring the absorbance of five Fe(oPH)2+3 standards at 510 nm. From that information, a calibration curve was plotted and used to find the amount of Fe2+ that was in two unknown water samples based on the absorbance readings obtained with them at 510nm. The equation of the line was found to be y=0.1765x + 0.0705. It was then determined that there was no iron present in water sample A, while for water sample B, the iron was present in the proportions of 0.9037ppm, 1.614x10-5M and 9.037x10-3%.
Introduction: Spectroscopy is the study of the interaction of light or electromagnetic radiation with matter. Spectrophotometry is any technique that uses light to measure chemical concentrations. Electromagnetic radiation is a form of energy when reacted with matter, can be absorbed, reflected or refracted, and how EMR reacts with matter depends on the properties of the material, based on the frequency, wavelength, absorbance etc. The electromagnetic spectrum shows representative molecular processes that occur when light in each region is absorbed. The visible spectrum spans the wavelength range 380-780nm, so each region is absorbs at different wavelengths.
The red-orange complex that forms between Iron (II) and 1,10-phenanthroline is useful for the determination of iron in water supplies. The reagent is a weak base that reacts to form phenanthrolinium ion in acidic media. The red-orange complex that forms between iron(II) and 1,10-phenanthroline (orthophenanthroline) is useful in determining iron in water supplies. The reagent is a weak base that reacts to form phenanthrolinium ion, phenH+, in acidic media.
A commonly used method for the determination of trace amounts of iron involves the complexation of Fe2+ with



References: Skoog and West, Fundamentals of Analytical Chemistry, 2nd Ed., Chapter 29. Vogel, A Textbook of Quantitative Inorganic Analysis, 3rd Ed., p. 294, 310 and 787.

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