of dialysis tubing, 25 mL of sucrose solutions with concentrations of 0.02 Molar, 0.04M, 0.06M, 0.08M, 1.0M, as well as 25mL of distilled water, six 250 mL beakers, a balance, and paper towels. We first cut the dialysis tubing into 6 pieces, each 1ft. long, and placed them into a beaker of water. We then tied off the dialysis tubing and poured 25 mL of distilled water in. We repeated this with the rest of the five pieces dialysis tubing, pouring a different molarity of sucrose solution in different dialysis tubes. After all the tubes were filled and tied, we then dried the bags and weigh each one on the scale. After all the data was recorded, we filled all the beakers about ¾ full of distilled water, placed the bags into each beaker in unison, and waited 30 minutes. Next, the bags were removed from the beakers, dried, and weighed separately. We expected the mass to increase with increasing molarity because with the higher the concentrations, more water would need to be diffused into the bag to reach…
If the four dialysis tubes containing different concentrations of sucrose are all placed in their appropriate solutions for 20 minutes, then my predictions are as follows: The 1% in 1% sugar solution will keep a constant weight and stay the same size. The 1% in 50% solution will shrink, due to the higher concentration of sugar outside of the bag. The 20% in 1% solution will swell, due to the higher concentration inside of the bag, and the 50% in 1% solution will do the same.…
The water in the test tubes surrounding the dialysis tubings was tested with standard food test reagents at the beginning of the experiment and again after one hour. The results showed that neither starch nor reducing sugar was present in the water surrounding tubes A and B at the beginning of the experiment. (7 marks)…
Exercise 1: Cell Transport Mechanisms and Permeability: Activity 1: Simulating Dialysis (Simple Diffusion) Lab Report…
What does the test of the jar water indicate? That the starch molecules in the dialysis tubing are larger than the in the tubing therefore they cannot be transferred through the process of osmosis or diffusion.…
Figure 1 shows the average amount of absorbance for each tube, containing different levels of pH. Tube 2 had an acidic pH level, Tube 3 had a neutral pH level, and Tube 4 had a basic pH level. It is indicated that the absorbance rates were the highest for the neutral pH level, with a final absorbance rate of 0.166. This was followed by a basic pH (0.106). The acidic pH level had the least amount of absorbance with a final absorbance rate of 0.069. This reinforces the idea that the ALP enzyme worked best under conditions with a neutral pH and worked the least in an acidic pH environment.…
A way to relate this to the real world is that people could use the concept of osmosis to make more accurate administering IVs to put into patients in hospitals. Osmosis is extraordinarily important in the biological processes where the solvent is water. This transport of water and molecules across the membranes is essential to many processes in living organisms and keeping them healthy. In general, this experiment helped understand the different ways osmosis works and how concentration can change the weight of…
Part 1 sought to measure the movement of water in response the addition of various concentrations of NaCl. It was hypothesised that as the concentration of NaCl increases, then the amount of lysis of the red blood cells would decrease. Part 2 aimed to determine how permeable red blood cell membranes were to various solutes. It was hypothesised that as the lipid solubility of solutes (Kether) increases, as does the permeability coefficient. Part 3 of the experiment aimed to produce and measure diffusion potentials across two different membranes of semi-permeability. It was hypothesised that as the voltage increases, as does the log of the concentration gradient.…
10) The distilled water would have a higher concentration of water molecules and would also have a higher water potential. The red blood cells would increase in size because water is moving from the area of higher water potential (the distilled water) to the area of lower water potential (the red blood cells) until dynamic equilibrium is reached.…
Explain what happened to the blood cells at the various levels of concentration. Be sure to refer to the solutions as being hypotonic, hypertonic and isotonic.…
1. What effect does increasing the afferent radius have on glomerular filtration rate and pressure?…
In part B, what caused the mass of the dialysis bags to change? Was there more or less water in the dialysis bags at the conclusion of the experiment? Explain.…
This experiment will identify the movement and exchange of water through semipermeable membranes and the recognizable amount of cellular destruction possible as a result in this change of environment. Tested were different samples of mammalian red blood cells (RBC) which were microscopically analyzed and measured for absorbency rates with a spectrophotometer when introduced to the various solute concentrations of 0.6% salt (Na), 0.897% Na, 5.0% Na, and distilled water with a 0.0% Na solute concentration. My hypothesis is that cellular form and contents will either change or maintain form and consistency when introduced to these various different environments as osmosis occurs until a dynamic equilibrium has been reached between the membrane and its environment. It was found that when RBC’s are introduced to various solute concentrates their form and consistency did changed with highest amount of hemolysis evident in the distilled water 0% Na concentration with an absorbency rate of 2.091% and the lowest amount of hemolysis occurring…
4. Red blood cells are placed in three beakers containing the following solutions: Beaker distilled water, Beaker B – isotonic solution, Beaker C – 5% salt solution. Describe the effect each solution will have on the cells and explain why.…
When conducting the osmosis lab, the initial tonicity of the cell was 0 grams. The beginning weight of the cell was 18.75 grams and…