Preview

Effect of Gene Treatment in C.Elegans Worms on Chemosensation

Powerful Essays
Open Document
Open Document
3094 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Effect of Gene Treatment in C.Elegans Worms on Chemosensation
The Effect of Srb-16 and F35B1.3 RNAi Gene Treatment on Chemosensation in Caenorhabditis elegans through Observation of Chemotaxis.
Abstract:
The study of sensory systems in humans is a vital area of study to further pursue our knowledge of the mechanisms occurring in the nervous systems. However due to the complications surrounding human testing, model organisms such as C. elegans worms can be utilized as a comparison to create paradigms relevant to human sensory systems as the genetic basis for both organisms is related amongst other factors such as the worm’s rapid reproductive cycle. Using RNAi treatment on C. elegans we tested the effects of inhibiting the synthesis of particular proteins in relation to the organism’s ability to detect and consequently move towards – that is chemosensation and chemotaxis - characteristically favorable odorants. This type of work hopes to build a basis for understanding the network of chemosensory signaling systems in humans and the corresponding proteins produced by particular gene sequences. Results suggested that gene sequence Srb-16 was not involved in encoding for a protein involved in chemosensation although contrary to previous experiments. The second gene sequence that was analyzed, F35B1.3- a mostly unknown sequence- was shown to likely encode for a protein in the sensory olfactory system as characteristic chemosensation behavior of C. elegans was altered.
Introduction:
This experiment was carried out to test the role of gene sequences Srb-16 and F34B1.3 in the chemosensation of C. elegans. Using RNAi treatment, the genes were inhibited from encoding their specific proteins and the resulting effect was analyzed to determine whether or not the genes play a role in chemotaxis of diacetyl. Diacetyl (DA) is a toxic buttery smelling odorant normally attractive to C. elegans. By observing the worms post RNAi treatment in their ability to detect and move towards DA, conclusions were drawn as to whether the gene sequence

You May Also Find These Documents Helpful

  • Satisfactory Essays

    Muscle Physiology Quiz

    • 1537 Words
    • 7 Pages

    c. stimulation of both taste and olfactory receptors interact to provide what we consider to be taste…

    • 1537 Words
    • 7 Pages
    Satisfactory Essays
  • Satisfactory Essays

    6. If the level of the odor-producing chemicals dissolved in the mucus surrounding the olfactory cilia reaches a threshold, a receptor potential and then an action potential will be generated and passed to the olfactory nerves in the olfactory bulb.…

    • 427 Words
    • 2 Pages
    Satisfactory Essays
  • Powerful Essays

    BIO 104 Chapter 3

    • 7229 Words
    • 29 Pages

    O P C C Human ribosome TRANSPORT PROTEINS Proteins involved in the movement of molecules across the cell membrane. FACILITATED DIFFUSION The process by which large or hydrophilic solutes move across a membrane from an area of higher concentration to an area of lower concentration with the help of transport proteins.…

    • 7229 Words
    • 29 Pages
    Powerful Essays
  • Good Essays

    2. signaling mechanisms first evolved in ancient prokaryotes and unicellular eukaryotes, then adopted for new uses…

    • 1160 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Nt1310 Unit 8

    • 418 Words
    • 2 Pages

    Neural cells in the nasal ways discover odorant molecules that bind to receptors on the external cell surface. Each olfactory nerve cell binds on only one odor. In this way, the brain sorts the smells by which group of nerves is signaling. Since scents are complex, many different molecules and neurons will fire together to signal the brain for analysis. The axons of the olfactory receptor cells send signal to terminate the glomeruli. In each glomerulus, receptor cells carry scent information to the mitral cells. These mitral cells refine the scent information and send them to the brain to process.…

    • 418 Words
    • 2 Pages
    Good Essays
  • Good Essays

    But, those fish embryos, like the Hudson River Tomcod, that possess a resistance to PCBs, are able to develop normally. Recently, scientists have discovered the type of adaptation that has allowed the Hudson River Tomcod to develop a resistance to theses contaminants and it has increased our understanding of the process of evolution. The receptor proteins, AHRs (aryl hydrocarbon receptors), which are present in the cytoplasm of cells of all vertebrates, play a large role in the Tomcod’s PCB resistance. PCBs, when diffused into cells, bind to the AHR proteins, and are carried into the nucleus, attaching to the cells DNA and activating genes that would normally not be activated. But, because of a mutation in the gene that codes for AHR, this pathway is blocked in the Hudson River Tomcod, preventing the PCBs from binding to the AHR proteins and prematurely activating genes. The mutation in the gene which encodes the AHR protein is a simple six base pair deletion, resulting in a protein that in only two amino acids short of the normal protein, which is made up of roughly 1000 amino acids in total. This random mutation led to a tremendously beneficial result for those Tomcods that live in a toxic environment, such as the Hudson…

    • 877 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Ebola Case Study

    • 739 Words
    • 3 Pages

    6) Who does dsRNA interact with Toll Receptor 3 and why is it found in the endosome? (10 pt)…

    • 739 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Dual Inner Observations

    • 1324 Words
    • 6 Pages

    The olfactory epithelium is located in the nasal passage and is lined by olfactory receptors, which consist of golf protein that can be stimulated by odor molecules. When stimulated, the golf protein stimulates the release of a cyclic AMP catalyzing enzymes. Upon catalyzing, the cyclic AMP acts as a transmitter signaling the opening of sodium ion channels resulting in depolarization of the receptor cells. Olfactory sensory information travels from the axon through the cribriform plate holes and mitral cell synapse. The mitral cells are located in the olfactory bulbs encompassing the olfactory tract.…

    • 1324 Words
    • 6 Pages
    Good Essays
  • Powerful Essays

    □ Linda Buck identified a large family of odorant receptor genes in rats(1,000 types); belong to the G protein…

    • 7457 Words
    • 30 Pages
    Powerful Essays
  • Better Essays

    Gajardo, Gonzalo M., Patrick Sorgeloos, and John A. Beardmore. "Abstract." National Center for Biotechnology Information. U.S. National Library of Medicine, 28 Nov. 2006. Web. 04 Apr. 2013. .…

    • 1427 Words
    • 6 Pages
    Better Essays
  • Better Essays

    amylase lab report

    • 1766 Words
    • 8 Pages

    Cited: Campbell, Neil A., Jane B. Reese, Martha R. Taylor, Eric J. Simon. Biology 105 Taken From; Biology: Concepts & Connections (Fifth Edition) Benjamin Cumming, San Francisco, CA, 2013.…

    • 1766 Words
    • 8 Pages
    Better Essays
  • Good Essays

    Unit 1 Gcse Biology

    • 4688 Words
    • 19 Pages

    receptors in the eyes that are sensitive to light receptors in the ears that are sensitive to sound receptors in the ears that are sensitive to changes in position and enable us to keep our balance receptors on the tongue and in the nose that are sensitive to chemicals and enable us to taste and to smell receptors in the skin that are sensitive to touch, pressure, pain and to temperature changes.…

    • 4688 Words
    • 19 Pages
    Good Essays
  • Satisfactory Essays

    Anatomy

    • 288 Words
    • 2 Pages

    * ofalction - the sense of smell, it involves olfactory receptors in paited olfactory organs responding to chemical stimuli…

    • 288 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    known to man. Just take a look at the apple iPhone. The iPhone now has Siri…

    • 493 Words
    • 2 Pages
    Good Essays
  • Satisfactory Essays

    Taste aversion helps with an organism’s survival. Taste aversion is when nausea is associated to a particular taste because that taste resulted in nausea. For example, there is a chemical/ drug that is used to treat substance abuse, and in the presence of alcohol makes you become violently ill. This makes alcoholics who are given the chemical/ drug learn to avoid drinking alcoholic beverages. To the alcoholic, there is an apparent link between the two stimuli. The alcoholics learn to associate alcohol and illness, and therefore stay away from any and all alcohol beverages as a way to ensure their…

    • 511 Words
    • 3 Pages
    Satisfactory Essays