Enzymes in Living Tissues
Enzymes in Living Tissues
Purpose:
The purpose of the lab was to analyze the enzymes in living tissues, represented by the pieces of liver. Also, hydrogen peroxide was used to demonstrate these effects.
Storyboard:
Materials:
2 50 mL beakers
10 mL graduated cylinder
3% hydrogen peroxide solution
Hot water bath
Lemon juice or HCl
Fresh liver
Forceps
Procedure:
Measure 10 mL of hydrogen peroxide and record its temperature.
Pick the liver up with the forceps and add it to the hydrogen peroxide, record the temperature once more.
Record the reaction.
Using the same piece of liver, repeat the same process once more.
Obtain a fresh piece of liver and place into boiling water for several minutes.
While waiting, place another piece of liver into a beak of acid, or lemon juice, and allow it to soak.
Retrieve the boiled piece of liver and allow to cool.
Meanwhile obtain 10 mL of fresh hydrogen peroxide and record the temperature.
Place cooked liver into the beaker of fresh hydrogen peroxide, record the reaction and temperature.
Remove liver and discard; rinse beaker.
Once more, obtain new hydrogen peroxide and place the acid soaked liver in the beaker. Record the reaction and temperature.
Clean all the equipment.
Gather all data from lab and make a table.
Data:
Type of Liver
Temperature of Liquid
Temperature After Liquid
Observations
Fresh with Hydrogen Peroxide
23°C
32°C
Quickly fizzed and developed bubbles; water turned a brown color, liver sank to bottom of beaker
Old with Hydrogen Peroxide
23°C
29°C
Less bubbles and fizz; liquid was more clear
Boiled
22°C
26°C
No reaction; liver was gray and hard; pieces fell off
Acid Soaked
24°C
24°C
Small amount of bubbles and fizz; liver floated to the top of the surface.
Conclusion:
The liver contains many
Purpose:
The purpose of the lab was to analyze the enzymes in living tissues, represented by the pieces of liver. Also, hydrogen peroxide was used to demonstrate these effects.
Storyboard:
Materials:
2 50 mL beakers
10 mL graduated cylinder
3% hydrogen peroxide solution
Hot water bath
Lemon juice or HCl
Fresh liver
Forceps
Procedure:
Measure 10 mL of hydrogen peroxide and record its temperature.
Pick the liver up with the forceps and add it to the hydrogen peroxide, record the temperature once more.
Record the reaction.
Using the same piece of liver, repeat the same process once more.
Obtain a fresh piece of liver and place into boiling water for several minutes.
While waiting, place another piece of liver into a beak of acid, or lemon juice, and allow it to soak.
Retrieve the boiled piece of liver and allow to cool.
Meanwhile obtain 10 mL of fresh hydrogen peroxide and record the temperature.
Place cooked liver into the beaker of fresh hydrogen peroxide, record the reaction and temperature.
Remove liver and discard; rinse beaker.
Once more, obtain new hydrogen peroxide and place the acid soaked liver in the beaker. Record the reaction and temperature.
Clean all the equipment.
Gather all data from lab and make a table.
Data:
Type of Liver
Temperature of Liquid
Temperature After Liquid
Observations
Fresh with Hydrogen Peroxide
23°C
32°C
Quickly fizzed and developed bubbles; water turned a brown color, liver sank to bottom of beaker
Old with Hydrogen Peroxide
23°C
29°C
Less bubbles and fizz; liquid was more clear
Boiled
22°C
26°C
No reaction; liver was gray and hard; pieces fell off
Acid Soaked
24°C
24°C
Small amount of bubbles and fizz; liver floated to the top of the surface.
Conclusion:
The liver contains many