Fruit Flies Experiment Research

At Banaras Hindu University in India zoologist scientist were working on research on the changes in stress and life traits in fruit flies. The scientists, Seema Sisodia, Puja Verma and Bashisth Narayan Sing explain there research in “The Effect of Diet Quality and Associated Metabolic Changes in Adult Stress Response and Life-History Traits in Drosophila Ananassae” The D. ananassae used in the experiment are flies collected from fruit and vegetable india in October 2010. The purpose of the study was to observe the role of diet during stress response at cellular and metabolic levels in fruit flies. Before the research began they first had to collect and harvest the flies for the experiment. Eggs were collected and once they had hatched and finished larvae development, the virgin flies aged until ready to be tested. The flies used had to be the same age and were maintained under the same laboratory conditions with different nutritional diets. This experiment would measure desiccation, starvation, chill-coma, heat shock, and egg-to-adult viability. This experiment is researching
Once the experiment started females and males were treated in groups of 20. A couple different methods were used for the statistical analysis of the data. This included the Kaplan-Meirs method, which was used to estimate the observed survival time. Then a log rank test was used to compare the two survival curves. Finally a two-way ANOVA is used to test the variation in stress response for different traits. These traits consisted of desiccation starvation, chill-coma recovery, heat shock survival, egg-to-adult viability and lipid content. The test is shown on eight different line graphs with the independent variables being one of the tests performed, such as starvation, and the dependent variable measuring the probability of