Hanging Drop Preparation Lab Report
SU_BIO2071_W1_A2_Alimole_. A.doc.
Exercise 1: Hanging-drop and wet-mount preparations
How does true motility differ from Brownian movement? Brownian movement is the result of molecules of the suspending water bumping into the bacterium. This is an asymmetrical, spasmodic movement, which gives cells a vibrating look. This does not show proper motility. Correct motility is movement in a specific constant direction that shows winding and spinning. What morphological structure is responsible for bacterial motility? Flagella are the morphological structure responsible for bacterial motility. These are semi-rigid structures used in moving microbial cells. They are attached to the cell through intricate proteins named the hook and basal …show more content…
Hanging drop preparation is very valuable because it allows for factual study ability. It offers extended observation abilities
What is the value of a wet-mount preparation?
The value of wet-mount preparation is that it allows surveillance of living cells to determine motility. This is made by placing the specimen in a drop of water on a microscope slide and covering it with a cover glass using no stain. It is best viewed with as little illumination as possible and must be done quickly because of drying. Due to the limited stability of Trichomonas, the wet mount is not a suggested test choice in an outpatient location where transportation of the specimen to the lab is involved
Exercise 2: Simple stains
Define acidic and basic dyes. What is the purpose of each?
Acidic dye also recognized as a negative stain, is defined as a negatively charged dye, containing single or extra acidic clusters, like a sulfur collection. On the other hand, basic dye is a positively charged dye which is typically synthetic and reacting with negatively charged atoms
What is the purpose of fixing a slide that is to be …show more content…
The hanging drop is the established technique for inspecting pristine microbes, while the wet mount is easier to complete and typically offers enough data.
List at least three types of bacteria whose names reflect their shapes and arrangements, and state the meaning of each name. • Streptococcus is spherical in shape and commonly seen in strep throat
• Lactobacillus is bacillus or rod shaped and found in probiotics
• Spirillum Pulli is spirillum or spiral shaped, and evident in rat bite fever
Exercise 3: Gram stain
What is the function of the iodine solution in the Gram stain? If it were omitted, how would staining results be affected?
Omitting the iodine in the gram's stain would mean, the crystal violet that goes into the cell would be incapable of forming the Crystal Violet Iodine (CVI) composite. The CVI complex molecule possesses a bigger size than that of Crystal violet molecule; therefore, gram-positive bacteria are able to maintain the stain.
What is the purpose of the alcohol solution in the Gram
Exercise 1: Hanging-drop and wet-mount preparations
How does true motility differ from Brownian movement? Brownian movement is the result of molecules of the suspending water bumping into the bacterium. This is an asymmetrical, spasmodic movement, which gives cells a vibrating look. This does not show proper motility. Correct motility is movement in a specific constant direction that shows winding and spinning. What morphological structure is responsible for bacterial motility? Flagella are the morphological structure responsible for bacterial motility. These are semi-rigid structures used in moving microbial cells. They are attached to the cell through intricate proteins named the hook and basal …show more content…
Hanging drop preparation is very valuable because it allows for factual study ability. It offers extended observation abilities
What is the value of a wet-mount preparation?
The value of wet-mount preparation is that it allows surveillance of living cells to determine motility. This is made by placing the specimen in a drop of water on a microscope slide and covering it with a cover glass using no stain. It is best viewed with as little illumination as possible and must be done quickly because of drying. Due to the limited stability of Trichomonas, the wet mount is not a suggested test choice in an outpatient location where transportation of the specimen to the lab is involved
Exercise 2: Simple stains
Define acidic and basic dyes. What is the purpose of each?
Acidic dye also recognized as a negative stain, is defined as a negatively charged dye, containing single or extra acidic clusters, like a sulfur collection. On the other hand, basic dye is a positively charged dye which is typically synthetic and reacting with negatively charged atoms
What is the purpose of fixing a slide that is to be …show more content…
The hanging drop is the established technique for inspecting pristine microbes, while the wet mount is easier to complete and typically offers enough data.
List at least three types of bacteria whose names reflect their shapes and arrangements, and state the meaning of each name. • Streptococcus is spherical in shape and commonly seen in strep throat
• Lactobacillus is bacillus or rod shaped and found in probiotics
• Spirillum Pulli is spirillum or spiral shaped, and evident in rat bite fever
Exercise 3: Gram stain
What is the function of the iodine solution in the Gram stain? If it were omitted, how would staining results be affected?
Omitting the iodine in the gram's stain would mean, the crystal violet that goes into the cell would be incapable of forming the Crystal Violet Iodine (CVI) composite. The CVI complex molecule possesses a bigger size than that of Crystal violet molecule; therefore, gram-positive bacteria are able to maintain the stain.
What is the purpose of the alcohol solution in the Gram