How Can It Be Used To Identify Trace Amounts Of Vanillin?

Abstract-Timur
Solid phase extraction is a sample preparation technique that overperform liquid /liquid extraction in its efficiency, labor intensity and waste disposal. The purpose of this lab is to learn how SPE can be used to identify trace amounts of Vanillin. Stock solution of vanillin in water with concentration of 50 ppm was prepared. Four solutions with concentration of 5 ppm, 1 ppm, 0.5 ppm and 0.1 ppm were made from stock solution. UV-Vis spectra of standard solution was recorded at 229 nm and equal to 0.544 AU. Vanillin was separated on SPE cartridges using a 50% methanol and 50% water as eluting solvent. UV-Vis of blank, 1 ppm and 0.1 ppm vanillin sample was obtained at 0.078 AU and 0.014 AU. 5 ppm solution of amlodipine besylate was made, its UV-Vis spectra were found to be 1.853 AU. Mixture of equal parts of amlodipine besylate and vanillin was prepared, Uv-Vis data detected at 0.120 AU..

Introduction-Riley The
Using SPE is useful in analytical chemistry. SPE pre-concentrates a sample that is being analysed so that it has a higher concentration of analyte. After SPE the sample is known as an analytical sample and the absorption spectra of an analytical sample is more accurate than that of a sample before preconcentration. Before pre-concentration the sample may cause spectral and chemical interference. SPE is useful to use when a scientist wants to extract a nonpolar analyte from a polar solution, or vice versa, the technique is very selective (5).
Is SPE helpful in all instances with any kind of sample?
No, SPE is not useful if your sample has an analyte that is polar in a polar solution or a nonpolar analyte in a nonpolar solution, there is not an SPE cartridge that will be able to separate the analyte from the solution.
What was the difference between your two vanillin loadings? The 1 ppm vs. the 0.1