How Does Chromatography Work?

Mobile Phase
The mobile phase is the phase that moves through the stationary phase (explain that stuff). As the mobile phase is moving through the stationary phase, it picks up compounds that need to be tested, taking those compounds with it. Through adsorption, the mobile phase separates into different components (soinc). The mobile phase can be liquid, gas, or supercritical fluid.
How Does Chromatography Work?
Chromatography is a surface effect. It works by first starting off with an unidentified liquid being injected into a column. As the chemicals in the liquid move down the column, they separate depending on their physical and chemical attributes, such as speed and weight.
To easily picture the process, the example will pertain to a paper chromatography process in which the liquid is moving over the paper, separating the mixture as it travels up the piece of paper. When the liquid mobile phase is applied to a solid, the separation starts off by the mobile phase moving past the solid. During this process, liquid molecules travel to the surface of the solid and stick there for a short amount of time before returning back to the liquid phase. This is called adsorption (not to be confused with absorption). This process continues as the liquid travels up the solid. Each
To start off, the mixture is injected into the mobile phase. A pump feeds the mobile phase through the column. The sample is then separated into different components as it travels down the column. This is dependant on which different chemicals will absorb into the stationary phase at varying times. After the sample is completely through the column, it is then released from the stationary phase where different chemicals will come out at different times due to the speed in which it travels through the stationary phase. This is dependent on the the attraction one chemical has to the column versus a different chemical