How Does Temperature Affect Peroxidase

Effect of Temperature on Peroxidase Ability to Break Down H2O2
By: Rodneika Crutcher
Abstract
Temperature affects the ability of peroxidase to break down hydrogen peroxide. In this experiment our professor extracted peroxidase from potato tissue. In order to determine how temperature affects peroxidase we created solutions and measured their absorbance levels after water bath treatments. The more absorbent the solution was the less hydrogen peroxide there was in the solution. This means the peroxidase was able to break down the hydrogen peroxide. The less absorbent the solution the harder it was for peroxidase to break down hydrogen peroxide.
Introduction
Enzymes are proteins that catalyze chemical reactions. This means that they lower the
After that take them out, mix the pairs and measure the absorbance the same way as before.

Results
Temperature does have an effect on peroxidase. As figure 1.1 shows, the temperature treatment affected the ability of peroxidase to break down hydrogen peroxide. The lower the temperature the more absorbent the solution was. This shows that at lower temperatures peroxidase breaks down hydrogen peroxide easier and faster. The warmer the temperature treatment was the less absorbent the solution was, thus showing that peroxidase couldn’t break down hydrogen peroxide as easily.
During the recovery experiment the solutions were returned to room temperature after their water bath treatment. In this experiment the absorbance levels were very close to one another, as shown in Figure 1.1. As a result we can come to the conclusion that peroxidase can still break down hydrogen peroxide when experiencing a temperature change. Figure 1.1 peroxidase breaks down H2O2 easier and faster at lower temperatures, but struggles to break down H2O2 at warmer