14. Regarding the effect of temperature on enzyme activity, what was the independent variable? Temperature…
In the lab with germinated and dry peas along with glass beads, determining the rate of respiration in each different type of seed was the goal. The hypothesis stated that since the germinated peas were still alive, they would contain a higher rate of respiration needed to remain healthy compared to the dried peas and glass beads.…
In experiment one, involving the rate of respiration of germinated peas when exposed to a variety of different temperatures. From a general standpoint the data collected matched the hypothesis formed, as the temperature increases the rate of respiration will increase as well. This was shown as the average ppm of oxygen was taken from each group. Comparing the data from the class is shows the effect of human error on the results. For example group #4 seemed to have back wards results this may have been due to an improper seal on the oxygen sensor.…
Enzymes are biological catalysts. They work by lowering the activation energy needed to initiate a chemical reaction. Enzymes work within an optimal temperature and optimal pH. Enzymes are highly specific for a single substrate. The Enzyme is usually much larger in size than the substrate it binds to. In some cases, an enzyme requires something called a cofactor to begin the chemical reaction. There were four different experiments that were executed in the enzyme lab. Experiment 7.1, the first experiment, was performed to test the effect of temperature on enzymatic activity. Based on what I know about the effect of temperature on the enzymatic activity of lactase, if the lactase used in today’s lab was extracted from human cells, I hypothesize that the optimal temperature for lactase to be in is around 37 degrees Celsius, which is the average human body temperature. The second experiment performed, experiment 7.2, tested the effect of pH on enzymatic activity. Based on what I know about the effect of pH on the enzymatic activity of lactase, if the lactase used in today’s lab was extracted from human cells, I hypothesize that the optimal pH level is 7, which is the average pH level in humans. Experiment 7.3 tests enzymatic specificity. Knowing that lactase is specific for the substrate lactose, lactase will only bind to lactose and not work with the other substrate used in this experiment, maltose. The last experiment of the lab, 7.4, was done to determine the cofactors of the enzyme, lactase. I hypothesize that EDTA, a cofactor inhibitor, will have a negative effect on the reaction and lower the amount of glucose that could possibly be produced if EDTA was not present.…
Hypothesis: The germinating beans will discharge more CO2 than the peas that are not germinating. Materials: 1250 mL respiration chamber Vernier CO2 gas sensor Vernier computer interface thermometer 25 non-germinating kidney beans 25, 24 hour germinating kidney beans 25, two hour warm germinating kidney beans 25, two hour cold germinating kidney beans Paper towels Safety Hazards: There are no need for goggles or gloves within this lab, but be aware of the choking hazard that the beans can cause. Procedure: Gather all materials Prepare a 100 mL beaker of beans/peas with warm water (warm germinating beans/peas), then prepare a 2nd 100 mL beaker with cold water instead of warm water (cold germinating beans/peas)…
1. Based upon your experimental results, write a paragraph discussing the relationship between environmental conditions and enzyme function.…
The objective of this lab was to figure out which has a higher cell respiration rate between crayfish and elodea. In order to figure this out we first set up three beakers to represent our control, elodea and crayfish and filled them with 75mL of culture solution which were dechlorinated making the solution acidic. We then had to place both the elodea and the crayfish in separate beakers filled with 25mL of water. The increase in volume of the water would represent the volume of the two test subjects. We then covered each beaker with plastic, but for the elodea we placed it under a can so it could be in the dark. After waiting 15 minutes to allow them to respire we took it out and add four drops of phenolphthalein, which was also acidic, to both beakers. Once both beakers got their four drops we added drops of NaOH, which was a base, until the solution turned pink. Our results were that the respiration rate of the crayfish was higher than the elodea.…
The purpose of this experiment is to design and conduct a basic scientific experiment, identify and control variables. Some other objectives that we learned is to write a formal hypothesis, identify the basic and integrated process skills of science that were used in this experiment. Also, we will learned the effects of temperature on goldfish respiration. This experiment will be conducted with four students , one goldfish, thermometer, a stop watch, and a beaker. Our results showed that the fish temperature increased and the fish breathing slowed down.…
In this experiment, water and potato enzymes were mixed to create a solution and placed into three test tubes. The first test tube was placed in 0 degrees Celsius, second test tube 22 degrees Celsius, and third in 95 degrees Celsius. The null hypothesis is when the temperature is changed; the concentration will not be affected. The alternate hypothesis is the more the temperature increases, the more the concentration decreases; as the temperature decreases, the concentration increases.…
Weight the mouse and use soda lime for mouse to perspire. Oxygen was inhaled and carbon dioxide was exhaled. Carbon dioxide was absorbed by soda lime. Rate of respiration was measured in terms of ml of oxygen per min over grams.…
3.Describe and explain the relationship between the amount of oxygen consumed and time? The amount of oxygen consumed was greatest in germinating peas in warm water. The oxygen consumption increased over time in germinating peas.…
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The purpose of this investigation is to figure out how temperature, pH, and enzyme concentration affect the reaction rate of an enzyme. It’s important to understand how certain factors affect enzymes because of their crucial role in the metabolic processes of life. Enzymes lower the activation energy necessary for a chemical reaction to occur, allowing vital processes such as digestion, ATP production, and DNA replication to occur efficiently. Using a Vernier Gas Pressure Sensor the rate of reaction of the enzyme catalase as it decomposed Hydrogen Peroxide in a test tube was measured and then results were put on the screen of a Macintosh computer using Logger Pro software and Vernier computer software. Several trials were then done with one factor, either temperature, pH, or enzyme concentration being altered, while the remaining two stayed constant. The rate of reaction of catalase should increase with enzyme concentration until a certain point until it reaches its limit. For temperature the rate of reaction of catalase should increase until the enzyme catalase denatures, and then after the rate should start to decrease. When exposed to different pH levels the rate of reaction of catalase should increase as the enzyme catalase reaches its optimal pH level, once it’s at that point the rate of reaction should then decrease as the pH level goes farther away from catalase’s optimal pH level. The data for the trials of pH and enzyme concentration support the expected hypotheses of how the reaction rate would be affected, however the data did not support the hypothesis made for the effects of temperature on an enzyme’s reaction rate due to divergent data from the expected results. (need this line or is conclusion and results of data and trends summarized with data supported hypotheses) ask if titel too long,,…
A: It is affected by temperature change, when the temperature rise it increase the rate of the anaerobic respiration, up to point. Soon the heat will start putting a lot of stress of the organism and then the rate of the anaerobic respiration will go down, more rising in temperature will eventually kill the organism.…
What was the effect of anaerobic respiration in different environments determined the increase in size of a dough by the temperature?…