Then, add 2-3 drops of hydrogen peroxide for each tube and wait for 5 minutes. Allow some time for the foaming reaction to occur and finish. Use a metric ruler to measure the thickness of the foam layer on the fluid surface of each test tube in millimeters (mm).
Record your results.
These methods are best when indicating how effective catalase is under different pH environments by how much foam is produced.
Obtain three test tubes and label each with the numbers 1, 2, and 3 with a glass-marking pen (preferably on a piece of masking tape) and place them in a test tube rack. Add a 0.5 cm cube of Beef liver into each tube (if not already prepared, use a scalpel or knife to cut your own pieces). Using a medicine dropper, add 3-4 drops of pH solution 2 for test tube 1, pH 7 for test tube 2, and pH 11 for test tube 3. Add a few drops of tap water for each tube.
Then, add 2-3 drops of hydrogen peroxide for each tube and wait for 5 minutes. Allow some time for the foaming reaction to occur and finish. Use a metric ruler to measure the thickness of the foam layer on the fluid surface of each test tube in millimeters (mm).
Record your results. These methods are best when indicating how effective catalase is under different pH environments by how much foam is produced.