Investigate the Factors That Affect the Rate of Respiration in Yeast
Mashrek International School
Investigate the Factors that Affect the Rate of Respiration in Yeast. (Temperature)
Fawzi El Ansari
Biology HL
Title: Investigate the Factors that Affect the Rate of Respiration in Yeast. (Temperature)
Aim: The aim of this experiment is to investigate the effect of changing the temperature on the rate of respiration in yeast. This will be done by placing equal amounts of yeast in each beaker that contains the same pH solution. Each beaker will be mixed with glucose solution and then will be placed at a different temperature in which the amount of CO2 produced will be measured every one minute.
Rationale: As we all know, respiration is a process that takes place in all living organisms where it converts sugar, taken in by the organism into energy. Its formula is C6H12O6+ 6O2----> 6CO2+ 6H2O + ATP. The yeast in this experiment will have to respire an-aerobically because there will be an absence of oxygen. The higher the temperature the more kinetic energy there is. When there is more kinetic energy, the molecules will move faster thus colliding with each other more often, so therefore there will be a higher percentage chance of an enzyme colliding with its substrate successfully. However, once optimum temperature is reached, the substrate can no longer bind to the active site, and the reaction will no longer take place. Here, the enzyme will begin to deform and the hydrogen bonds formed between amino acids will break, and the yeast enzyme will begin to denature.
Hypothesis: If the temperature increases, then the rate of the reaction will increase and the rate of CO2 will increase until it reaches its maximum temperature and then begins to denature.
Variables:
Independent Variable: Temperature of yeast solution
Dependent Variable: Rate at which CO2 is released by time
Controls: pH of solution kept at 7 Type of concentration of glucose sugar used Time set of experiment for all trials is 10 minutes Mass of yeast set at 4
Investigate the Factors that Affect the Rate of Respiration in Yeast. (Temperature)
Fawzi El Ansari
Biology HL
Title: Investigate the Factors that Affect the Rate of Respiration in Yeast. (Temperature)
Aim: The aim of this experiment is to investigate the effect of changing the temperature on the rate of respiration in yeast. This will be done by placing equal amounts of yeast in each beaker that contains the same pH solution. Each beaker will be mixed with glucose solution and then will be placed at a different temperature in which the amount of CO2 produced will be measured every one minute.
Rationale: As we all know, respiration is a process that takes place in all living organisms where it converts sugar, taken in by the organism into energy. Its formula is C6H12O6+ 6O2----> 6CO2+ 6H2O + ATP. The yeast in this experiment will have to respire an-aerobically because there will be an absence of oxygen. The higher the temperature the more kinetic energy there is. When there is more kinetic energy, the molecules will move faster thus colliding with each other more often, so therefore there will be a higher percentage chance of an enzyme colliding with its substrate successfully. However, once optimum temperature is reached, the substrate can no longer bind to the active site, and the reaction will no longer take place. Here, the enzyme will begin to deform and the hydrogen bonds formed between amino acids will break, and the yeast enzyme will begin to denature.
Hypothesis: If the temperature increases, then the rate of the reaction will increase and the rate of CO2 will increase until it reaches its maximum temperature and then begins to denature.
Variables:
Independent Variable: Temperature of yeast solution
Dependent Variable: Rate at which CO2 is released by time
Controls: pH of solution kept at 7 Type of concentration of glucose sugar used Time set of experiment for all trials is 10 minutes Mass of yeast set at 4