The composition of kefir grains is variable and not well defined. It is described as a symbiotic association between lactic and acetic bacteria and yeast. Kefir is an acid, viscous, slightly carbonated dairy beverage [Garrote et al., 2001], and related to a variety of health benefits [McCue & Shetty, 2005 & Rodrigues et al., 2005]. Traditionally kefir grains have been used for centuries in many countries, for example, in Tibet, China, as the natural starter in the production of the unique self-carbonated dairy beverage [Saloff-Coste, 199]. Kefir grains contain a complex microbial symbiotic mixture of lactic acid bacteria (Lactobacillus, Lactococcus, Leuconostoc, and Streptococcus spp.), and yeasts (Kluyveromyces, Candida, Saccharomyces and Pichia) [Farnworth, 2005 & Witthuhn et al., 2005]. Ricotta cheese is a high moisture soft cheese [Modler & Emmons, 2001]. It can be produced using cheese whey or milk, or a mixture of both [Pizzillo et al.,2005]. Ricotta cheese is very mild and it is used in many Italian dishes. Fresh Ricotta cheese had a mild and muty flavour and is used as a flavor enhance in Salad [Kosikowski, 1982]. Several methods have been suggested for Ricotta cheese making [Weatherup, 1986 and Modler & Emmons, 1994]. These include study the effect of type of acidulant on the quality of Ricotta cheese. Making ricotta like cheese with kefir grains, which yeasts and lactic acid bacteria coexist in a …show more content…
The following microbiological analyses were performed: (i) determination of total aerobic counts on nutrient agar (Oxoid) at 30°C for 48 h, (ii) coliforms on MacConkey agar (Oxoid) by incubation at 30°C for 24 h, (iii) yeasts and molds by incubation on malt agar (Oxoid) (pH adjusted to 4.5 by sterile solution of 10% lactic acid) at 30°C for 48 h, and (iv) staphylococci by incubation on Staphylococcus agar No. 110 medium (Oxoid) at 37°C for 48h [Atlas,