Microbiology Lab Session 1 Observing Bacteria and Blood
Session #1 Lab 1 OBSERVING BACTERIA AND BLOOD
D Haynes
BIO-218A Microbiology Lab
Friday, January 17, 5:00pm
Objective(s): Our objective is to gain knowledge of the functions and operations of the microscope through observation of bacteria and blood slides that are available for this assignment. We are also learning to “see” and observe various bacteria and their shapes as well as live yogurt cultures, blood, plaque and cheek swab samples and their arrangements. We are to gain knowledge by observing blood and its cells so to be able to distinguish between blood cultures and bacteria specimens.
Procedure(s) : General procedures are as follows:
Gathering and setting up experiments, hands on operation of the microscope and its various lenses, manipulating specimens on and off the microscope and following and carrying out viewing instructions for slide, regular and oil immersion and various staining and wet mount techniques.
Observations/Results :
Sample
Observations
Part 1
Part 2
150x
600x
1500x (Oil Immersion)
Amoeba proteus
Appears to be oval with a tail-like end and solid as well as membrane layered cells.
The cells appear to be fuzzy and not well defined
Appears to look like the sun with the inner cell having multiple textures.
Anabaena
A paisley shaped cell with bubbles in the membrane with a hollow center cell
Several spherical shapes with pink, yellow and hollow centers
An oval shaped cell with yellow and red linings.
Ascaris Eggs
Dozens of eggs appear most look yellow some appear clear.
The eggs appear to be gell-like. Some are even folded.
I can see the outer ring of the cell along with tiny circles inside the cell.
“e” slide
Appears to be an E with fuzzy outer layer
I can see a small part of the “E” and the fuzzy outline appears clear.
I can see only grey with specks here and there.
Paramecium
Appear to be divided with nucleus centers.
I can see the division
D Haynes
BIO-218A Microbiology Lab
Friday, January 17, 5:00pm
Objective(s): Our objective is to gain knowledge of the functions and operations of the microscope through observation of bacteria and blood slides that are available for this assignment. We are also learning to “see” and observe various bacteria and their shapes as well as live yogurt cultures, blood, plaque and cheek swab samples and their arrangements. We are to gain knowledge by observing blood and its cells so to be able to distinguish between blood cultures and bacteria specimens.
Procedure(s) : General procedures are as follows:
Gathering and setting up experiments, hands on operation of the microscope and its various lenses, manipulating specimens on and off the microscope and following and carrying out viewing instructions for slide, regular and oil immersion and various staining and wet mount techniques.
Observations/Results :
Sample
Observations
Part 1
Part 2
150x
600x
1500x (Oil Immersion)
Amoeba proteus
Appears to be oval with a tail-like end and solid as well as membrane layered cells.
The cells appear to be fuzzy and not well defined
Appears to look like the sun with the inner cell having multiple textures.
Anabaena
A paisley shaped cell with bubbles in the membrane with a hollow center cell
Several spherical shapes with pink, yellow and hollow centers
An oval shaped cell with yellow and red linings.
Ascaris Eggs
Dozens of eggs appear most look yellow some appear clear.
The eggs appear to be gell-like. Some are even folded.
I can see the outer ring of the cell along with tiny circles inside the cell.
“e” slide
Appears to be an E with fuzzy outer layer
I can see a small part of the “E” and the fuzzy outline appears clear.
I can see only grey with specks here and there.
Paramecium
Appear to be divided with nucleus centers.
I can see the division