Purpose: Cells produce toxic wastes, in this experiment hydrogen peroxide, and without some sort of molecule to break it down the cell will die, along with the organism itself. However with the aid of an enzyme, catalase, hydrogen peroxide is able to be broken down into an intermediate and thereafter harmless substances water and oxygen. The goal of this lab is to measure the reaction rate of this process in different substances such as a liver, a vegetable, and breast tissue. By using variables such a pH and temperature we are able to how the rate of reaction is altered or improved. If it has improved, the optimum has been discovered and the enzyme will create a higher reaction rate. If above the optimal points, proteins will denature and the reaction rate will remain the same. This is vital for cellular activity for if homeostasis is not reached enzymatic activity will decrease or the enzymes will simply denature and the toxicity within the cell will increase killing the cell.…
Enzymes are special proteins that carry out chemical reactions, also known as catalysts. Two important features that make all enzymes catalysts are their ability to bind to a substrate. A substrate is anything that needs to be changed into something else. The second important feature is that it works to lower the activation energy without being used or changed in the reaction (Hudon-Miller, 2012.…
Enzymes are proteins that act as catalysts during a biochemical process. Catalysts are non-changing enzymes that can increase or decrease activation energy to accelerate or slow down a biochemical reaction without using additional energy.…
Enzymes are biological catalysts (substances that speed up a chemical reaction without themselves being permanently altered)…
Enzymes are protein, they are used to catalyse metabolisms in all organisms. They break down complex molecules and build up complex molecules from simple molecules, these two processes are catabolic reaction and anabolic reaction respectively. Enzymes are needed in these two processes to catalyse releasing and taking up ATP molecules.…
The enzyme peroxidase has been shown to break down H2O2. Enzymes are known to increase the rate at which a chemical reaction occurs. We looked at factors that affected the breakdown of hydrogen peroxide. These effects are the different temperatures and pH levels the enzymes were placed in. We found that the optimum, or best condition, temperature for the enzymes tested was about 22 degrees Celsius. The optimum pH level for the enzyme was 7.…
a. Enzyme: biological catalysts that regulate chemical reactions that occur in cells. Responsible for things such as converting food to energy, replace old damaged tissues, disposal of cellular waste products. Are responsible for lowering the activation energy.…
Alternate Hypothesis 1: I there is an increase in concentration in concentration of Hydrogen Peroxide then the reaction rate of the liver catalase will increase.…
Catalysts are substances that increase chemical reactions while using less energy. They also remain unchanged after use, which enables it to be used repeatedly. Cells have special catalysts called enzymes, which are specialized proteins that help accelerate chemical cell reactions. (Evert, RF & Eichhorn, SE 2013). Enzymes also control plant metabolic processes such as respiration (Evert RF, Eichhorn SE & Perry JB 2013). This experiment focuses on the enzyme catalase. Catalase breaks down hydrogen peroxide into water and oxygen. Hydrogen peroxide is a waste product of cell metabolism that can be toxic to the cell (Evert RF, Eichhorn SE & Perry JB 2013).…
Preparation Step 1: Research (online) the reaction between catalase and hydrogen peroxide and be familiar with the reactants, products, and enzyme. You will include the overall reaction in your lab report for this experiment. We will measure enzyme activity by measuring the height of the bubbles produced.…
Figure 1: The reaction rates of enzyme-catalysed reactions of hydrogen peroxide into diluted water depending of the varying percentage concentrations of catalase. Mean rates (±SE) of 5 separate experiments (n=5) are shown. Line of best fit (y = 0.0002x + 0.0038 R² = 0.9154) is presented using the trend line function in Excel.…
Enzymes are biological molecules that catalyze chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical reactions in a biological cell need enzymes in order to occur at rates sufficient for life. Like all catalysts, enzymes work by lowering the activation energy for a reaction, thus dramatically increasing the rate of the reaction. As a result, products are formed faster and reactions reach their equilibrium state more rapidly. Most enzyme reaction rates are millions of times faster than those of comparable un-catalyzed reactions. As with all catalysts, enzymes are not consumed by the reactions they catalyze, nor do they alter the equilibrium of these reactions. However, enzymes do differ from most other catalysts in that they are highly specific for their substrates. Enzymes are known to catalyze about 4,000 biochemical reactions.…
In this laboratory exercise we studied enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment.…
Enzymes are a key aspect in our everyday life and are a key to sustaining life. They are biological catalysts that help speed up the rate of reactions. They do this by lowering the activation energy of chemical reactions (Biology Department, 2011).…
Since the enzyme lowers the activation energy, the rate of reaction increases without consuming enzyme. In this experiment, the substrate is hydrogen peroxide.…