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Many organisms can decompose hydrogen peroxide (H2O2) enzymatically. Enzymes are globular proteins, responsible for most of the chemical activities of living organisms. They act as catalysts, substances that speed up chemical reactions without being destroyed or altered during the process. Enzymes are extremely efficient and may be used over and over again. One enzyme may catalyze thousands of reactions every second. At the start of the reaction, there is no product, and the concentration is the same as the atmosphere. After a short time, oxygen accumulates at a rather constant rate. The slope of the curve at this initial time is constant and is called the initial rate. As the peroxide is destroyed, less of it is available to react and the O2 is produced at lower rates. When no more peroxide is left, O2 is no longer produced. Objectives Use a computer and an Oxygen Gas Sensor to measure the production of oxygen gas as hydrogen peroxide is destroyed by the enzyme catalase at various enzyme concentrations. Measure and compare the initial rates of reaction for this enzyme when different concentrations of enzyme react with H2O2. hypothesis Write a 2-part hypothesis that predicts what you think will happen in this investigation and a scientific reason. MATERIALS computer w/ Logger Pro software1.5 H2O2Vernier computer interface - LabProliver enzyme suspension Vernier O2 Gas Sensor10 mL graduated cylinder250 mL Nalgene bottleclock or stopwatch2 pipettes labeled Diagram of procedure procedure Plug in the LabPro to an outlet, then connect the Oxygen Gas Sensor to the LabPro. Connect the LabPro to a computer and prepare the computer for data collection by opening the file 06A Enzyme (O2) from the Biology with Computers folder of Logger Pro. (Macintosh HD(Applications(Logger Pro 3(Experiments(Biology with Computers) Add 6 mL of 1.5 H2O2 to the 250 mL Nalgene bottle. Using a pipette, add 5 drops of enzyme suspension to the Nalgene bottle. Begin timing

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