Top-Rated Free Essay
Preview

osmosis in onion cell

Good Essays
743 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
osmosis in onion cell
Date: 28/11/13
EVALUATION OF THE EFFECT OF SODIUM CHLORIDE ON ONION EPIDERMAL CELLS

INRODUCTION
A living plant cell will shrinks or swells depending on the solute concentration of the cell in relation to the solute concentration of the fluid surrounding the cell (1). It follows that water will move from a region of high water concentration to a region of low water concentration therefore, if a cell is placed in a hypertonic solution water will move from the cell into the solution until the cell shrinks(1). Further water loss causes can cause the cells protoplasm to peel away from the cell wall leaving a gap between the cell wall and the cell membrane, a process called plasmolysis(1) However if the cell is placed in a hypotonic solution, water will move from the solution into the cell and cause the cell to burst (1).
A cell in an isotonic solution neither swells nor shrinks because there is no concentration gradient for water across the cell membrane (1)
The objective of this experiment is to find out the effect distilled water, 3% sodium chloride solution and 5% sodium chloride solution have on onion epidermal cells.
From the above literature, its hypothesised that the cells in distilled water should maintain their shape, cells in 3% sodium chloride should shrink and the cells in 5% sodium chloride should experience more shrinking than that of the 3% solution.
The onion epidermal cell is transparent with a simple structure so it is a suitable cell for studying the effect of water loss on cells (2) Some specimens can be viewed directly underneath the microscope but putting a drop of water on the specimen can improve how the structures appear under microscope and also prevent the specimen from drying out on the slide (2). Adding water to the specimen is called wet mount.
The liquid used in wet mount fills the space between the slides to support the specimen and also allows light to pass through easily (3).
The liquid is usually water can also be a stain.
Stains are used in microscopy to highlight structures in biological structures for viewing under microscopes (3). There are many types of stains each is used for its own benefits, iodine, eosin are some of the common stains used in the microscopy (3). Iodine is used as a cell stain because it’s absorbed by cell organelles which are mostly transparent and turn them coloured making them more visible under the microscope (3).
It was found that the cells which were placed in 3% solution increased in size contrary to the hypothesis. The cells placed in 5% solution shrank and cells in placed in normal saline kept their normal shape.

MATERIALS
Glass slide
Cover slip
Onion tissue
Dropper
Light miscrope
Iodine
Sodium chloride (NaCl)
Distilled water
Tweezers
Measuring tube
Bunsen tube
Pipette
Blotting paper
Stage micrometer

METHOD
Lay down three different slides labelling the three different solution you intend to use.
Peel a thin layer of tissues (epidermis) using the forceps (tweezers) from the whole onion.
Put the epidermis on the center of each of the slide displayed and labelled.
Drop a few drops of each of the labelled solutions onto the onion membrane, making sure the tissues is flattened and not wrinkled on the slide.
Wait for 3 minutes before putting a cover slip on the slide.
Put the cover slip on the stained tissue and the different solutions involve.
Gently tap out any air bubbles and try to soak out any extra solution around the covered tissues.
Adjust the diaphragm so that the right amount of light is shining on the slide stage.
Put the slide onto the microscope stage and fix with the stage clips
Observe the tissues under the microscope under low, medium and high powers. Also making sketches, writing down observation.
Making sure of an eye piece graticule and stage micrometer scale to measure cells and units.
Be sure to write what magnification for each sketch.
Make sure you label any organelles you see. Cell wall, membrane, nucleus and cytoplasm
Calculate magnification of drawings and actual sizes of specimens

DATA
The eye piece graticule remains constant no matter what magnification the epidermis are looked at.

RESULTS

DISCUSSIONS

CONLUSION

References
1. Sperelakis, Nicholas (2011). Cell Physiology Source Book: Essentials of Membrane Biophysics. Academic Press. p. 288. ISBN 978-0-12-387738-3.
2. Horobin RW, Kiernan JA (2002) Conn 's Biological Stains. A Handbook of Dyes Stains and Fluorochromes for Use in Biology and Medicine. 10th ed. Oxford: BIOS. ISBN 1-85996-099-5
3. Clark G (1981) Staining Procedures, 4th ed., Baltimore: Williams & Wilkins, p. 412, ISBN 0683017071.

References: 1. Sperelakis, Nicholas (2011). Cell Physiology Source Book: Essentials of Membrane Biophysics. Academic Press. p. 288. ISBN 978-0-12-387738-3. 2. Horobin RW, Kiernan JA (2002) Conn 's Biological Stains. A Handbook of Dyes Stains and Fluorochromes for Use in Biology and Medicine. 10th ed. Oxford: BIOS. ISBN 1-85996-099-5 3. Clark G (1981) Staining Procedures, 4th ed., Baltimore: Williams & Wilkins, p. 412, ISBN 0683017071.

You May Also Find These Documents Helpful

  • Good Essays

    3. When a cell is placed in a hypertonic solution, what happens to the cell? shrivels…

    • 726 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Biolab 1208 Lab Report

    • 840 Words
    • 4 Pages

    Introduction: The biological membranes are composed of phospholipid bilayers, each phospholipid with hydrophilic heads and hydrophobic tails, and proteins. This arrangement of the proteins and lipids produces a selectively permeable membrane. Many kinds of molecules surround or are contained within cells, but water is perhaps the single most important molecule in any living system (Hayden and McNeil 2012). Since water molecules are so small, they are constantly going into and out of the cell. Osmosis is a situation where more water molecules are moving across the membrane in one direction than the other (Hayden and McNeil 2012). During osmosis the net movement of water molecules will be from a solution that has a lower osmotic concentration to a solution that has a higher osmotic concentration. When a solution has a higher concentration of solute within the cell than out, it is called hypertonic. When a solution has a lower concentration of solute within the cell than out, it is called hypotonic. And when there are equal concentrations inside and out of the cell, it is called isotonic. The relative osmotic concentration can be determined by a change in mass of the tissue.…

    • 840 Words
    • 4 Pages
    Better Essays
  • Satisfactory Essays

    bio potato lab

    • 368 Words
    • 1 Page

    7. Humans' cells are in isotonic solution that keeps equal number of solutes both inside and outside of the cell. If the cell is put in a hypotonic solution, water will move into the cell. This causes the cell to burst and die. On the other side, if you put the cell in a hypertonic solution, water will move the outside of…

    • 368 Words
    • 1 Page
    Satisfactory Essays
  • Satisfactory Essays

    EARLY CELLS

    • 341 Words
    • 1 Page

    When any type of plant cell is placed in water the cell wall expands to prevent cell membraneto collapse , because of the osmosis produce in this.…

    • 341 Words
    • 1 Page
    Satisfactory Essays
  • Good Essays

    Rubber Egg Lab

    • 893 Words
    • 3 Pages

    2. Explain your observations in detail in terms of concentration gradient, diffusion, osmosis, osmotic pressure, passive transport, and active transport.…

    • 893 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Scientific Method and Egg

    • 475 Words
    • 2 Pages

    Cells are affected by the water through diffusion. You see if you when you apply or remove water from the cell it will most likely show you how it functions. For example when you have the egg and you apply a bit of water it will most likely shrink if you add a lot of water it’s mostly likely that the egg will burst.…

    • 475 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Cells must move materials through membranes in order to maintain homeostasis. The cellular environment is aqueous, indicating that the solutes dissolve in the solvent, water. When a cell is hypertonic, or hypotonic, to its surroundings, it tries to make concentration of solution inside and outside itself equal. However, the solutes are too big to pass the cell membranes without the help of channel proteins, or transport proteins. Water may freely pass through the membrane by osmosis, which requires no energy. Thus, the cell starts to take in, or release, water until it is isotionic to its…

    • 468 Words
    • 2 Pages
    Good Essays
  • Good Essays

    mocking bird

    • 620 Words
    • 3 Pages

    hypertonic, plasmolysis : hypertonic solution has a higher solute concentration than the cytosol of a cell. In a hypertonic solution a plant cell will lose water and shrink away from the cell wall, a process called plasmolysis.…

    • 620 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Ap Bio Lab One

    • 432 Words
    • 2 Pages

    This lab helps further our understanding of essential principles behind diffusion and osmosis. Permeability, concentration gradients, plasmolysis, water potential, and equilibrium were also concepts that were delved into in this lab. Understanding how diffusion and osmosis works is essential to understanding biology. Each time a cell has something move into or out of it, some sort of principle studied in this lab is occurring. Diffusion, osmosis, and passive and active transport are all fundamental concepts of Biology. This lab simulated osmosis in the cell. In this case, we used dialysis tubing to simulate the semi-permeable membrane of an animal cell.…

    • 432 Words
    • 2 Pages
    Good Essays
  • Powerful Essays

    Physio 9.0 Lab 1:3

    • 972 Words
    • 4 Pages

    Exercise 1: Cell Transport Mechanisms and Permeability: Activity 3: Simulating Osmotic Pressure Lab Report Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. Which of the following is true of osmosis? You correctly answered: c. It is a type of diffusion. 2. Which of the following occurs when a hypertonic solution is added to cells? You correctly answered: d. The cells shrink. 3. The variable that affects osmotic pressure is You correctly answered: a. the concentration of nondiffusing solutes. 4. The net movement of water would be into the cell in a You correctly answered: b. hypotonic solution.…

    • 972 Words
    • 4 Pages
    Powerful Essays
  • Powerful Essays

    also when cutting to be sure to use the same ruler and making sure the…

    • 2078 Words
    • 9 Pages
    Powerful Essays
  • Good Essays

    Why are plant and animal cell affected differently by it? A: When the solute concentration is different, the total water entering or exiting the cell produces a force called osmotic pressure. Animal cells shrink because they only have a cell membrane while plant cells don’t shrink because they have a cell membrane and a cell…

    • 627 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Microbiology

    • 656 Words
    • 3 Pages

    There are three types of environments in which cells are located which include isotonic, hypotonic and hypertonic. In an isotonic environment, the amount of water and solute are the same both inside and outside of the cell. As water drifts into the a cell, the same amount flows out creating a balanced environment both inside and outside of the cell. When there is a high level of water on the outside of the cell and a high amount of solute inside the cell, water will be drawn inside of the cell creating a hypotonic solution. The increase in water inside the cell causes the cell to become engorged and erupt. In a hypertonic environment, water will leave the cell, as the amount of water in the cell is higher than that outside of the cell. As a cell looses water in a hypertonic environment it becomes smaller in size and withers. Isotonic environments are preferred by most microbes for the most advantageous growth, although some live in slightly hypotonic solutions. (Alonzo, 2008)…

    • 656 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Active Transport

    • 368 Words
    • 2 Pages

    21. Hypertonic solution- a solution in which water molecules are moving out of a cell, causing it to shrink…

    • 368 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Osmosis Lab Report

    • 2530 Words
    • 11 Pages

    INTRODUCTION: In order to fully understand the concept of this experiment, one must first be introduced to diffusion. Diffusion is a spontaneous process by which molecule particles move from one area that is highly concentrated to another area in which its concentration is lower. Cells contain fluids and are surrounded by fluids; in order for a cell to function it is required to be in a balanced state. The progress in which a cell is in its balanced state is called equilibrium. Diffusion is a functioning way for cells to reach their equilibrium. Equilibrium is reached by controlling what enters and exits the cell through a cell membrane that selectively filters molecules by slowing down their movement, allowing them to pass through, or not allowing them to pass through the membrane. Diffusion and osmosis go hand-in-hand. Osmosis is generally the same as diffusion, however, deals solely with water. Osmotic pressure is the pressure of a solution against a semi-permeable membrane to prevent water from flowing into the membrane. In this lab, we are going to study tonicity; tonicity is the measure of this osmotic pressure and is the differential of pressure between two solutions separated by a selective membrane. To help identify the relative concentrations of solute particles of different solutions, we must understand that there are three possible differences in concentrations between a cell and its environment. The terms hypotonic, hypertonic, and isotonic are used in referring to the identification of the possible relative concentrations. The first term, hypotonic, is the solution that contains lower concentrations of solute particles, which means that the concentration inside the cell is greater than the concentration outside. A hypotonic solution causes the cell to swell in size. The second term, hypertonic, is the solution that contains higher concentration of solute…

    • 2530 Words
    • 11 Pages
    Good Essays