Paper Chromatography Lab Report
Chromatography Analysis of Black Pens to Determine Unknown Sample
Purpose:
Paper chromatography was performed on five different black pens, using four different solutions to determine which would be most appropriate to use on an unknown sample. Paper chromatography was then performed on the unknown sample to identify which pen was used to create it.
Procedure:
Each person participating in the analysis was assigned one solution to work with: V. Temple used distilled water, D. Sellers used a 20% ethanol solution, J. Chadwick used a 50% ethanol solution and J. Sanchez used a 90% ethanol solution. Each participant used the same procedure with their assigned solution, repeating it for each of five ink samples. The pens used were a Sanford Vis a vis, a Sharpie fine point, a Dixon Visaid, a Sharpie calligraphy and …show more content…
Blue=0.973
Yellow=0.822
Red=0.726
90% ethanol Purple=0.662
Green=0.455
Brown=0.195
Purple=0.853 Yellow=0.123 Blue=0.971
Yellow=0.797 Purple=0.76
Blue=0.347
Aqua=0.147
Red=0.053
Unknown Sample/
Solution B-Evidence 124 distilled water Blue=.961
Red= .684
Yellow= .513
50% ethanol Blue=.973
Red=.723
Yellow=.671
Questions:
Q1 - As a result of chromatography testing it was determined that the ransom note was written with a Sanford Vis a vis pen.
Q2 - Some solvent systems worked better than others because of the nature of the different types of chemicals used in the inks. Inks made with more polar substances will be more attracted to the water as a solution and inks made with more non-polar substances will be more attracted to ethanol as a
Purpose:
Paper chromatography was performed on five different black pens, using four different solutions to determine which would be most appropriate to use on an unknown sample. Paper chromatography was then performed on the unknown sample to identify which pen was used to create it.
Procedure:
Each person participating in the analysis was assigned one solution to work with: V. Temple used distilled water, D. Sellers used a 20% ethanol solution, J. Chadwick used a 50% ethanol solution and J. Sanchez used a 90% ethanol solution. Each participant used the same procedure with their assigned solution, repeating it for each of five ink samples. The pens used were a Sanford Vis a vis, a Sharpie fine point, a Dixon Visaid, a Sharpie calligraphy and …show more content…
Blue=0.973
Yellow=0.822
Red=0.726
90% ethanol Purple=0.662
Green=0.455
Brown=0.195
Purple=0.853 Yellow=0.123 Blue=0.971
Yellow=0.797 Purple=0.76
Blue=0.347
Aqua=0.147
Red=0.053
Unknown Sample/
Solution B-Evidence 124 distilled water Blue=.961
Red= .684
Yellow= .513
50% ethanol Blue=.973
Red=.723
Yellow=.671
Questions:
Q1 - As a result of chromatography testing it was determined that the ransom note was written with a Sanford Vis a vis pen.
Q2 - Some solvent systems worked better than others because of the nature of the different types of chemicals used in the inks. Inks made with more polar substances will be more attracted to the water as a solution and inks made with more non-polar substances will be more attracted to ethanol as a