poster presentation coursework
INTODUCTORY PLANT PATHOLOGY C123P1
Isolation & Study of
Sclerotinia (Monilinia) Fructigena
Extraction of
Polygalacturonase(PG) enzyme
Assay
Sclerotinia (Monilinia) Fructigena
• 10g of infected tissue was taken and ground in a mortar and pestle with
100Mm PH5 citrate buffer then filtered through 4 layers of muslin.
• Half of this mixture was boiled at
50°C for 10 minutes and the other half was left at room temperature.
• The above steps were repeated with a sample with healthy tissue.
• These solutions were then poured into separate wells o a sodium polypectate solution agar plate along with two controls of PG and boiled PG.
• This was left for incubation at 37C for 24 hours.
• The plates were then flooded with
2m HCL to precipitate any sodium polypectate out of solution.
• The agar plate was examined against a dark surface to measure any zones of clearance around cell wall. Sometimes known as Brown Rot, Monilinia fructigena is a plant pathogen. "Sclerotinia fructigena. a, the conidial form known as
Monilia." -Whitney, 1911
Fig 1.1 apple infected with S.Fructigena
The fungus Sclerotinia fructigena is a plant pathogen which causes brown rot disease in apples, peaches and some other fruits, characterized by comparative firmness of the invaded tissue. This fungus often infects the fruits through wounds caused by birds and wasps before the fruits are harvested.
Experimental Objective
To discover more about the fungus Sclerotinia (Monilinia) fructigena including :
1. Method to isolate pure samples.
2.To look at pure samples of the fungus microscopically and learn to identify them morphologically.
3.To determine whether the fungus is resistant to certain fungicides, for this experiment, a class of fungicides, the benzimidazoles (the MBCs) is used.
4.To determine whether the fungus has any polygalacturonase activity and whether this enzyme is affected by boiling.
5.To look at re-inoculation of the fungus,
Isolation & Study of
Sclerotinia (Monilinia) Fructigena
Extraction of
Polygalacturonase(PG) enzyme
Assay
Sclerotinia (Monilinia) Fructigena
• 10g of infected tissue was taken and ground in a mortar and pestle with
100Mm PH5 citrate buffer then filtered through 4 layers of muslin.
• Half of this mixture was boiled at
50°C for 10 minutes and the other half was left at room temperature.
• The above steps were repeated with a sample with healthy tissue.
• These solutions were then poured into separate wells o a sodium polypectate solution agar plate along with two controls of PG and boiled PG.
• This was left for incubation at 37C for 24 hours.
• The plates were then flooded with
2m HCL to precipitate any sodium polypectate out of solution.
• The agar plate was examined against a dark surface to measure any zones of clearance around cell wall. Sometimes known as Brown Rot, Monilinia fructigena is a plant pathogen. "Sclerotinia fructigena. a, the conidial form known as
Monilia." -Whitney, 1911
Fig 1.1 apple infected with S.Fructigena
The fungus Sclerotinia fructigena is a plant pathogen which causes brown rot disease in apples, peaches and some other fruits, characterized by comparative firmness of the invaded tissue. This fungus often infects the fruits through wounds caused by birds and wasps before the fruits are harvested.
Experimental Objective
To discover more about the fungus Sclerotinia (Monilinia) fructigena including :
1. Method to isolate pure samples.
2.To look at pure samples of the fungus microscopically and learn to identify them morphologically.
3.To determine whether the fungus is resistant to certain fungicides, for this experiment, a class of fungicides, the benzimidazoles (the MBCs) is used.
4.To determine whether the fungus has any polygalacturonase activity and whether this enzyme is affected by boiling.
5.To look at re-inoculation of the fungus,
Cited: William Dwight Whitney The Century Dictionary: An Encyclopedic Lexicon of the English Language (New York, NY: The Century Co., 1911) WILLETTS, H. J. and CALONGE, F. D. (1969), SPORE DEVELOPMENT IN THE BROWN ROT FUNGI (SCLEROTINIA SPP.)