Pre-Transfusion Blood Tests
Pre-transfusion Blood Tests:
Title: To perform the following tests: ABO and RH grouping using the Diamed Gel Card system. Rh and Kell phenotyping (antigen typing) using the Diamed Gel Card system. Direct Coombs Test (DCT) using the conventional tube system. Direct Coombs Test (DCT) using the Diamed Gel Card system. Antibody Identifications (IAT) technique using the conventional tube system. Antibody Identifications (ETC) technique using the Diamed Gel Card system.
Name: S. Ward
Date: 8/11/2012
Introduction:
The objective of this practical is to perform some pretransfusion tests, using various methods. Pretransfusion testing is carried out in all hospital blood bank laboratories and is used to minimise the risk of encurring a haemolytic transfusion reaction. Haemolytic transfusion reactions occur when a patient is transfused with red cells which have a foriegn antigen on the cell surface that the patient has an antibody to. While the ABO and Rh blood group systems are the most antigenic and thus can cause severe haemolytic transfusion reactions. The other blood group systems can also cause a (less severe) haemolytic reaction, these reactions can be fatal and so the procedures to avoid them are of great importance.
Materials & Method:
Not all tests were performed by all members of the class. ABO and Rh blood grouping using the Diamed Gel Card system; performed. Rh and Kell trying using the Diamed Gel Card system; not performed. Direct Coombs Test (DCT) using the Diamed Gel Card system;not performed. Antibody Identification (ETC) technique using the Diamed Gel Card system; performed but not centrifuged so no results were obtained. Antibody Identifications (IAT) technique using the conventional tube system; performed Direct Coombs Test (DCT) using the conventional tube system; performed.
Cell 1% suspensions were prepared for the bench from a 3% cell suspension provided.
Results:
As well as
Title: To perform the following tests: ABO and RH grouping using the Diamed Gel Card system. Rh and Kell phenotyping (antigen typing) using the Diamed Gel Card system. Direct Coombs Test (DCT) using the conventional tube system. Direct Coombs Test (DCT) using the Diamed Gel Card system. Antibody Identifications (IAT) technique using the conventional tube system. Antibody Identifications (ETC) technique using the Diamed Gel Card system.
Name: S. Ward
Date: 8/11/2012
Introduction:
The objective of this practical is to perform some pretransfusion tests, using various methods. Pretransfusion testing is carried out in all hospital blood bank laboratories and is used to minimise the risk of encurring a haemolytic transfusion reaction. Haemolytic transfusion reactions occur when a patient is transfused with red cells which have a foriegn antigen on the cell surface that the patient has an antibody to. While the ABO and Rh blood group systems are the most antigenic and thus can cause severe haemolytic transfusion reactions. The other blood group systems can also cause a (less severe) haemolytic reaction, these reactions can be fatal and so the procedures to avoid them are of great importance.
Materials & Method:
Not all tests were performed by all members of the class. ABO and Rh blood grouping using the Diamed Gel Card system; performed. Rh and Kell trying using the Diamed Gel Card system; not performed. Direct Coombs Test (DCT) using the Diamed Gel Card system;not performed. Antibody Identification (ETC) technique using the Diamed Gel Card system; performed but not centrifuged so no results were obtained. Antibody Identifications (IAT) technique using the conventional tube system; performed Direct Coombs Test (DCT) using the conventional tube system; performed.
Cell 1% suspensions were prepared for the bench from a 3% cell suspension provided.
Results:
As well as