The second lab deals with the chromatography of food dyes which means you figure out what kind of compounds are used in a product like food color etc. by comparing the result to FD&C Food Dyes. You use six different food dyes, four food colors out of the store, two different Kool Aid powder and four different colors of candy like M&Ms you use for the chromatograph. At the end you try to find which food dyes are in the other products.…
Make four pencil dots along this line 0.5 cm apart. Underneath each dot, label the marker that will be tested. Use the markers to draw a different colored dot on each of the pencil marks on the paper strip. Allow the ink to dry, and then add more color to each dot. make a prediction of the pigment components you expect to see from…
In this Laboratory experiment, my lab partner Alexander and I were able to understand thoroughly the physical and chemical properties of salt (NaCl) and sand (SiO2). Followed by the right procedure we were able to design and test out the components of both NaCl and SiO2 in order to separate the unknown mixture that we were able to find out about. We were also given the task to provide the percent composition of the mixtures. Therefore, the separation of components among this experiment allowed us to obtain the right numbers for our calculations.…
2) Based on maximum absorbance of the dye tested, select the appropriate wavelength on spectrometer.…
The purpose of this laboratory investigation is to observe how chromatography can be used to separate mixtures of a solution.…
2. In chromatography, components of a mixture distribute themselves between the stationary phase and the mobile phase. Explain how the components can be separated with these two phases.…
Tryptophane is the largest amino acid. Also two larger amino acids are Alanine and Serine. The largest Rf is Tryptophane. The polarity is like the Serine but the Alanine is nonpolar.…
5. What observations would lead you to believe that the ink is actually a mixture?…
Note: Please post this assignment to your dropbox. THE DROPBOX IS ANAGALOGOUS TO AN E-MAIL ACCOUNT. (To accomplish your assignment, you may cut&paste the observations section of the assignment into your text editor (such as Microsoft Word). Complete your answers - then send the answers as an attachment to the dropbox. Attachment examples include Word document, Excel graph, etc.) Your dropbox can be entered via the dropbox tab at the top of the screen. Make sure you title your assignments properly. You do not need to show your calculations. Edit the observations sheet to present the data in a clear fashion. USE CAPITAL LETTERS FOR THE TEXT/DATA YOU ENTER IN YOUR REPORT.…
Paper chromatography uses selective adsorption on a strip of paper. It is used for separating and identifying mixtures that are or can be colored, especially pigments. This can also be used in secondary or primary colors in ink experiments.…
4. It is important to cover the beaker so moisture doesn’t get into the beaker. It is important to not jostle the breaker because when you move the beaker the water moves to messing up the pigments.…
Background: Chromatography is a process that is used to separate a substance into its component parts. The separation occurs between the stationary and moving phase of the lab. The moving phase consists of a fluid and the stationary phase consists of a solid. The mixture we are trying to split up is integrated into the mobile phase. When the mobile phase interacts with the solid phase, the components of the mixture are attracted to the solid phase in varying degrees. Components with higher levels of attraction for the mobile phase result in a faster speed of transport throughout the solid phase. Components with higher levels of attraction for the solid phase result in a slower speed of transport throughout the solid phase. These differing levels of attraction end up in separation of the mixture into component bands, which exit the system as distinct liquids.…
A low distance means the molecules are absorbed to the stationary phase; the cellulose in the chromatography paper more than the solvent. The fraction of time spent in the mobile phase is the Rf value. Example- For the red candy there is one spot. Measuring from the middle of the spot to the spotting line the distance is .5cm this is the distance moved by the substance.…
Background: Bacteria is a prokaryotic cell that reproduces using the process of binary fission. Binary fission is “The process in which a parent cell splits into two daughter cells of approximately equal size”(northwestern.edu). The reproduction of bacteria can be stopped using antimicrobials. Antimicrobials were created and are used “to destroy disease-causing microbes”(cdc.gov). That means that antimicrobials fight and destroy organism such as bacteria.…
Thin Layer Chromatography or TLC is a technique used as a separation and identification technique. There are many forms of chromatography, but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase. In the case of TLC the stationary phase is the silica gel on the TLC tray.…