Saponifiable Lipids Fr
=========== BIOCHEMISTRY ============
Biochemistry Laboratory - CH 601L (2011-2012) Experiment 6
Characterization of Saponifiable Lipids
Kevin Arven A. Benin*, and Gabrielle Marie A. Bugna
Department of Chemistry, College of Science
University of Santo Tomas, Espana Street, Manila 1050
Date Submitted: August 25, 2011
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Abstract: The experiment was about the characterization of a fat/oil sample through three tests, namely the grease-spot test, saponification test, and unsaturation test. The procedures include the use of a filter paper divided into four sections and a drop of vegetable oil in a section; the same was done on lecithin, dichloromethane, and water and was heated. Oil, fat, and water were subjected into a saponification test using NaOH, and acidification using concentrated H2SO4. And lastly, oil, fat and glycerol were subjected into an unsaturation test by adding dichloromethane, and was added with a 5% Bromine-dichloromethane solution, the total volume of the 5% Br-DCM solution was then obtained.
Keywords:
Lipids, Saponification
1. Introduction Lipids are a group of the essential biomolecules in which are generally characterized by their insolubility in water, and are soluble in ethers, alcohols, and other fat solvents (Hawk, 1954). Lipids are classified into three classifications, simple, compound or conjugated, and derived lipids. Simple lipids are esters of fatty acids with various alcohols. Compound or conjugated lipids are esters of fatty acids containing groups in addition to an alcohol and a fatty acid. Derived lipids are those derived from simple/compound lipids through hydrolysis (Harper et al., 1977). Christie (2010) defined lipids as fatty acids and their derivatives, and substances related biosynthetically or functionally to these compounds. In
Biochemistry Laboratory - CH 601L (2011-2012) Experiment 6
Characterization of Saponifiable Lipids
Kevin Arven A. Benin*, and Gabrielle Marie A. Bugna
Department of Chemistry, College of Science
University of Santo Tomas, Espana Street, Manila 1050
Date Submitted: August 25, 2011
________________________________________________________________________
Abstract: The experiment was about the characterization of a fat/oil sample through three tests, namely the grease-spot test, saponification test, and unsaturation test. The procedures include the use of a filter paper divided into four sections and a drop of vegetable oil in a section; the same was done on lecithin, dichloromethane, and water and was heated. Oil, fat, and water were subjected into a saponification test using NaOH, and acidification using concentrated H2SO4. And lastly, oil, fat and glycerol were subjected into an unsaturation test by adding dichloromethane, and was added with a 5% Bromine-dichloromethane solution, the total volume of the 5% Br-DCM solution was then obtained.
Keywords:
Lipids, Saponification
1. Introduction Lipids are a group of the essential biomolecules in which are generally characterized by their insolubility in water, and are soluble in ethers, alcohols, and other fat solvents (Hawk, 1954). Lipids are classified into three classifications, simple, compound or conjugated, and derived lipids. Simple lipids are esters of fatty acids with various alcohols. Compound or conjugated lipids are esters of fatty acids containing groups in addition to an alcohol and a fatty acid. Derived lipids are those derived from simple/compound lipids through hydrolysis (Harper et al., 1977). Christie (2010) defined lipids as fatty acids and their derivatives, and substances related biosynthetically or functionally to these compounds. In
References: Christie, W. W, (2010). About Lipids. Retrieved from http://lipidlibrary.aocs.org/lipids.html. August 22, 2011. Harper, H. (1977). Review of Physiological Chemistry. Lange Medical Publications. USA Hawk, P. B. (1954). Practical Physiological Chemistry. Maple Press Company. USA Millo, F.R., and Loffredo, W. M. (1994). Qualitative Testing for Lipids in Molecular Laboratory Program /in Chemistry. Chemical Education Resources. Palmyra, Pennsylvania, USA. Retrieved from http://www.cerlabs.com/experiments/10875404472.pdf. August 22, 2011. http://www.fromnaturewithlove.com/resources/sapon.asp. Retrieved August 22, 2011 http://journeytoforever.org/biofuel_library/fatsoils/fatsoils2.html. Retrieved August 22, 2011 _______________ *Corresponding author