Standard curve analysis for DNA Quantities
Name:
ZHENG CHENGWEI
Partners:
Karen Chang
Mayra Perez
Mandeep Sablok
Di Zhang
Lab Section:
Lab Report 1
Biology 21 (1225)
Date:
09/21/2012
Result
The purpose of the experiment is to measure the amount of DNA extracted from a strawberry. In the experiment, we measured the absorbance of different concentrations of Methylene Blue. The data collected by our group is presented on Figure 1 and the average absorbance measured by whole class is stated on Figure 2. Table 3 shows the measurement on the amount of strawberry DNA extracted.
In Figure 1 and Figure 2, the data will be presented Standard Curve Graph. The relationship between the concentration of Methylene Blue and the absorbance is expected to be a direct proportion.
Figure 1: Standard Curve of light absorbance by ten different concentrations of Methylene Blue. The data of the figure is collected by our group Mitochondria. The relationship between two varieties is a direct proportion. The equation for the trend line is Y = 20.788 X - 0.0093 ; R ² = 0.9675.
This equation will be used to calculate the unknown concentration of Methylene Blue solution.
Figure 2: Standard curve of Average light absorbance by ten different concentration of Methlene Blue. The data is collected from all the groups in the class. All the points in the graph represent the means of all the groups.
The figure shows a direct proportion. Its equation for trend line is Y = 23.569 X – 0.0043; R ² = 0.991.
Table 1: The measurement of DNA extracted from strawberry. The total amount of DNA recover is measured and calculated using the standard curve made by my group. First, the absorbance of Methylene blue stained DNA so- lution is measured by spectrophotometer. Then, the concentration of solution can be figured out by using the trend line equation. Further, 0.001% Methylene Blue corresponds to 0.086mg/ml. Concentration is obtained by the conversion. Finally, the total amount of
ZHENG CHENGWEI
Partners:
Karen Chang
Mayra Perez
Mandeep Sablok
Di Zhang
Lab Section:
Lab Report 1
Biology 21 (1225)
Date:
09/21/2012
Result
The purpose of the experiment is to measure the amount of DNA extracted from a strawberry. In the experiment, we measured the absorbance of different concentrations of Methylene Blue. The data collected by our group is presented on Figure 1 and the average absorbance measured by whole class is stated on Figure 2. Table 3 shows the measurement on the amount of strawberry DNA extracted.
In Figure 1 and Figure 2, the data will be presented Standard Curve Graph. The relationship between the concentration of Methylene Blue and the absorbance is expected to be a direct proportion.
Figure 1: Standard Curve of light absorbance by ten different concentrations of Methylene Blue. The data of the figure is collected by our group Mitochondria. The relationship between two varieties is a direct proportion. The equation for the trend line is Y = 20.788 X - 0.0093 ; R ² = 0.9675.
This equation will be used to calculate the unknown concentration of Methylene Blue solution.
Figure 2: Standard curve of Average light absorbance by ten different concentration of Methlene Blue. The data is collected from all the groups in the class. All the points in the graph represent the means of all the groups.
The figure shows a direct proportion. Its equation for trend line is Y = 23.569 X – 0.0043; R ² = 0.991.
Table 1: The measurement of DNA extracted from strawberry. The total amount of DNA recover is measured and calculated using the standard curve made by my group. First, the absorbance of Methylene blue stained DNA so- lution is measured by spectrophotometer. Then, the concentration of solution can be figured out by using the trend line equation. Further, 0.001% Methylene Blue corresponds to 0.086mg/ml. Concentration is obtained by the conversion. Finally, the total amount of