Temperature And Enzymes
What effect does temperature have on the oxygen production of enzymes in an animal’s liver? A temperature which is too high would be able to produce little to no oxygen because of the extreme temperature. Just like a temperature too high, a temperature which is too low would also produce little to no oxygen for the same reason. Animals that have livers are considered warm-blooded, with very warm temperatures; therefore it would make sense for a warm temperature to produce the most oxygen; but one that is not too hot. If the temperature of the animal liver is 104° as opposed to 32°, 75°, 95°, and 109° (Fahrenheit), then there would be a higher oxygen production rate. Using devices that measure oxygen as well as cow catalase, several test tubes
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containing 2 drops of the catalase were heated and cooled to the temperatures 32°, 75°, 95°, 104°, and 109°(Fahrenheit). They were then poured into separate flasks and their oxygen production was measured after the devices that measure oxygen were placed inside to the flask accordingly. Each individual temperature was tested multiple times in order to insure as accurate data as possible.
The temperature that produced the highest rate of oxygen was the enzyme with a temperature of 104° It produced an average of 11.12% oxygen for the cow liver. This means that the catalyst functioned better than any other and the amount of oxygen produced was much more than anything else produced. 104°F was tested because it was the average temperature of the cow itself, and the fact that it produced the most amount of oxygen made the most sense. The averages of all of the other enzymes were all less than the average of the enzyme which was 104°F; therefore, the 104°F enzyme produced the most oxygen. Hence an enzyme with a very warm temperature would work the most effectively and produce the most oxygen because the enzyme with a very warm temperature produced the most oxygen.
There were a few flaws in the experiment which if not noticed and resolved immediately, would damage the results of the experiment.
For example, the oxygen sensor did not give the exact percentage of oxygen unless given enough time to process the contents of the flask. If the percentages were taken as soon as it was inserted into the flask, then the experiment would be extremely inaccurate. In addition to this, the amount of time taken to transport the test tubes from the location where it was heated/cooled and the location where the testing took place was far enough to where the test tube could heat up/ cool down in the process of transportation. In order to fix it (or at least minimize the damage done), the transportation time was reduced as best as possible. One error that was made had to do with the measuring of the catalase. It was not measured correctly for the first few trials and had to be redone. Another problem that arose was the amount of time given in comparison to the amount of trials required. It was time-consuming at first, but a process was established after some time and the work became very efficient. One limitation of the experiment was the fact that not all cows have a temperature of 104°, and if the highest functioning temperature of the enzyme had a correlation to the average temperature of the cow, then it wouldn’t be as accurate as possible. This could’ve been prevented by testing temperatures that are very close to 104°, but the amount of levels of IV would then become too high and the amount of time it would take to test it would be far too much. This information can be useful in the real world as well. If a cow or any warm-blooded animal had noticeable sickness, the temperature can possibly be the issue and action should be taken to insure that if that is the case then the amount of oxygen produced returns back to normal again. Further study can be done on this topic, as this experiment didn’t technically determine whether the
best-functioning temperature and the average temperature of the animal tested had any form of correlation. This can also be tested with different types of animal catalase.
containing 2 drops of the catalase were heated and cooled to the temperatures 32°, 75°, 95°, 104°, and 109°(Fahrenheit). They were then poured into separate flasks and their oxygen production was measured after the devices that measure oxygen were placed inside to the flask accordingly. Each individual temperature was tested multiple times in order to insure as accurate data as possible.
The temperature that produced the highest rate of oxygen was the enzyme with a temperature of 104° It produced an average of 11.12% oxygen for the cow liver. This means that the catalyst functioned better than any other and the amount of oxygen produced was much more than anything else produced. 104°F was tested because it was the average temperature of the cow itself, and the fact that it produced the most amount of oxygen made the most sense. The averages of all of the other enzymes were all less than the average of the enzyme which was 104°F; therefore, the 104°F enzyme produced the most oxygen. Hence an enzyme with a very warm temperature would work the most effectively and produce the most oxygen because the enzyme with a very warm temperature produced the most oxygen.
There were a few flaws in the experiment which if not noticed and resolved immediately, would damage the results of the experiment.
For example, the oxygen sensor did not give the exact percentage of oxygen unless given enough time to process the contents of the flask. If the percentages were taken as soon as it was inserted into the flask, then the experiment would be extremely inaccurate. In addition to this, the amount of time taken to transport the test tubes from the location where it was heated/cooled and the location where the testing took place was far enough to where the test tube could heat up/ cool down in the process of transportation. In order to fix it (or at least minimize the damage done), the transportation time was reduced as best as possible. One error that was made had to do with the measuring of the catalase. It was not measured correctly for the first few trials and had to be redone. Another problem that arose was the amount of time given in comparison to the amount of trials required. It was time-consuming at first, but a process was established after some time and the work became very efficient. One limitation of the experiment was the fact that not all cows have a temperature of 104°, and if the highest functioning temperature of the enzyme had a correlation to the average temperature of the cow, then it wouldn’t be as accurate as possible. This could’ve been prevented by testing temperatures that are very close to 104°, but the amount of levels of IV would then become too high and the amount of time it would take to test it would be far too much. This information can be useful in the real world as well. If a cow or any warm-blooded animal had noticeable sickness, the temperature can possibly be the issue and action should be taken to insure that if that is the case then the amount of oxygen produced returns back to normal again. Further study can be done on this topic, as this experiment didn’t technically determine whether the
best-functioning temperature and the average temperature of the animal tested had any form of correlation. This can also be tested with different types of animal catalase.