The Antipasmodic Activity of Molave Tree (Vitex Parviflora) Bark Extract in Albino Mice
The Antipasmodic Activity of Molave Tree (Vitex parviflora) Bark Extract in
Albino Mice
A Research Paper
Presented to Mrs. Ferrera Oira
In Partial Fulfillment of the Requirement in Research II
Georgette Mae P. Dineros
Jone Zelita C. Orlina
Anna Dominique G. Humbe
IV- Analysts Andromeda
Ramon Teves Pastor Memorial
Dumaguete Science High School
SY 2012-2013
ABSTRACT
Diarrhea is a common condition that affects people of any age and of any gender. It is characterized by frequent bowel movements. This causes dehydration and loss of salts. It may be caused by poor sanitation and contaminated food, milk or water. When diarrhea strikes, the first solution in mind would usually be antidiarrheal drugs. So the researchers thought of an alternative remedy for diarrhea. In this study, the researchers compared the effects of Molave tree Bark to the effects of antidiarrheal drugs on diarrhea. The preparation of extract was done by crushing 10 mg. of Molave Tree bark mixed with 10 mL distilled water. The bark was strained and the extract was considered our 100% crude extract. The researchers got the 75% and 50% plant extracts by mixing 0.75 mL of 100% plant extract to 0.25 mL distilled water and 0.5 mL of 100% plant extract to 0.5 mL distilled water respectively. For the control, 2 mg. antidiarrheal drugs were mixed with 2 mL distilled water. After starving 12 albino mice for 12 hours, each mouse received 0.2 mL of their respective extract. After one hour, each mouse was given 0.2 mL charcoal meal which is composed of 20 mL castor oil, 10 mL coconut oil and 2 g activated carbon. After another hour, the mice were all sacrificed by chloroform and dissected to get the intestines. The distance traveled by the charcoal was divided by the total length of the intestines and data was subjected to statistical tests.
ACKNOWLEDGEMENT
The researchers would like to thank, first of all, to God Almighty who gave us the
Albino Mice
A Research Paper
Presented to Mrs. Ferrera Oira
In Partial Fulfillment of the Requirement in Research II
Georgette Mae P. Dineros
Jone Zelita C. Orlina
Anna Dominique G. Humbe
IV- Analysts Andromeda
Ramon Teves Pastor Memorial
Dumaguete Science High School
SY 2012-2013
ABSTRACT
Diarrhea is a common condition that affects people of any age and of any gender. It is characterized by frequent bowel movements. This causes dehydration and loss of salts. It may be caused by poor sanitation and contaminated food, milk or water. When diarrhea strikes, the first solution in mind would usually be antidiarrheal drugs. So the researchers thought of an alternative remedy for diarrhea. In this study, the researchers compared the effects of Molave tree Bark to the effects of antidiarrheal drugs on diarrhea. The preparation of extract was done by crushing 10 mg. of Molave Tree bark mixed with 10 mL distilled water. The bark was strained and the extract was considered our 100% crude extract. The researchers got the 75% and 50% plant extracts by mixing 0.75 mL of 100% plant extract to 0.25 mL distilled water and 0.5 mL of 100% plant extract to 0.5 mL distilled water respectively. For the control, 2 mg. antidiarrheal drugs were mixed with 2 mL distilled water. After starving 12 albino mice for 12 hours, each mouse received 0.2 mL of their respective extract. After one hour, each mouse was given 0.2 mL charcoal meal which is composed of 20 mL castor oil, 10 mL coconut oil and 2 g activated carbon. After another hour, the mice were all sacrificed by chloroform and dissected to get the intestines. The distance traveled by the charcoal was divided by the total length of the intestines and data was subjected to statistical tests.
ACKNOWLEDGEMENT
The researchers would like to thank, first of all, to God Almighty who gave us the
Bibliography: Diarrhea. The New International Standard Medical and Health Encyclopedia. Volume 2.