Bio 430
Dr. Allen
Spray it or wipe it!!!
The scientific study on the effectiveness of CiDecon® Plus Wipes vs. CIDECON ® Q Aerosol on a mixed culture of bacteria (Serratia marcescens Staphylococcus epidermidis) living on publicly used door knobs
Fateema Thomas
Andrea Donaldson
Aryan Jaimangal
Tiffany McNeil
John Posey
Abstract Germs are a mist in every corner of the world. They are stealth and literally, are on everything. This can range from your cooking ware, or maybe you’re doorknobs, and even your fingertips. But exactly what can did mean for our human race? Are these bacterium good or bad? Bacterium sometimes can affect us in positive and negative lights. We can use this to our advantage in terms of symbioses. Some bacteria can cause disease, while some can fight them off and enhance your immune system. Staphylococcus epidermidis and Serratia marcescens are both bacterium that are known to do good, but also bad for the body. This is why aseptic technique is so important in our everyday life. But, which is the best method? In current decade, sprays and wipes, have lead the way in the innovation of disinfectants. So our group decided to base our expirement on this sole idea, which sterilization is more accurate, disinfectant wipes or spray? We cultured a mix of S. epidermidis and S. marcescens as our control. For the most part, the control flourished and we were ready to start testing. We hypothesized the spray would disinfect with a greater percentage than the wipes. We conducted four trails of placing the bacterium mix on 3 styles of door handles. Since the doors knobs had two sides, we would place the same control on both sides of the knob and disinfect each side with different disinfectants. There were intervals of 4 minutes, in which we let the bacteria sit, and swabbed the bacteria off. Immediately after, we inserted the sample on a nutrient agar dish. Finally, we incubated the samples for approximately 2