Thin Layer Chromatogragahy Lab Report
--- Experiment 1 : Thin-layer Chromatogragaphy : Analysis of Drug Components
CHEM221
Date experiment performed : January 20, 2014Date Submitted : January 27, 2014
Introduction
This laboratory has its first objective to familiarize with the thin layer chromatography (TLC) technique. Second, each student has to identify the unknown components by comparing it to one of the tree dissolved liquid analgesics: Acetaminophen, Aspirin and Caffeine. Finally, calculating the Rf value by measuring the mobile and stationary phase.
Chromatography is a technique used to separate mixtures into their compounds. It’s based on a mobile phase and a stationary phase. The mobile phase moves up the thin layer paper by taking the compounds with it, till it’s absorbed and stops moving. [1] The paper chromatography (silca gel) is a very uniform absorbent paper. Different components travel at different rates. Depending on the affinity each component has, it will move faster or slower up the TLC. To visualize the location of the different spots, an ultraviolet lam is necessary.
Results …show more content…
These organic compounds are colorless, however, by looking with UV light the spots can be observing. As the Figure 1,0 : thin layer chromatography representation down below, the spots are showed :
Figure 1.0 : thin layer chromatography representation
The first spot is the Caffeine, the second is the Aspirin, the third is the Acetoaminophen and the last is the unknown. The distance each compound have achieved a certain distance which have an important relation with the polarity of the molecule. The explanation will be explained in the discussion.
Table1.0 : Results for caffeine, aspirin, acetoaminophen and the unknown 150
Distancecomponent (±0,05cm) Distancesolvent (±0,05cm) Rf (±0,1)
Caffeine 1,20 6,00
CHEM221
Date experiment performed : January 20, 2014Date Submitted : January 27, 2014
Introduction
This laboratory has its first objective to familiarize with the thin layer chromatography (TLC) technique. Second, each student has to identify the unknown components by comparing it to one of the tree dissolved liquid analgesics: Acetaminophen, Aspirin and Caffeine. Finally, calculating the Rf value by measuring the mobile and stationary phase.
Chromatography is a technique used to separate mixtures into their compounds. It’s based on a mobile phase and a stationary phase. The mobile phase moves up the thin layer paper by taking the compounds with it, till it’s absorbed and stops moving. [1] The paper chromatography (silca gel) is a very uniform absorbent paper. Different components travel at different rates. Depending on the affinity each component has, it will move faster or slower up the TLC. To visualize the location of the different spots, an ultraviolet lam is necessary.
Results …show more content…
These organic compounds are colorless, however, by looking with UV light the spots can be observing. As the Figure 1,0 : thin layer chromatography representation down below, the spots are showed :
Figure 1.0 : thin layer chromatography representation
The first spot is the Caffeine, the second is the Aspirin, the third is the Acetoaminophen and the last is the unknown. The distance each compound have achieved a certain distance which have an important relation with the polarity of the molecule. The explanation will be explained in the discussion.
Table1.0 : Results for caffeine, aspirin, acetoaminophen and the unknown 150
Distancecomponent (±0,05cm) Distancesolvent (±0,05cm) Rf (±0,1)
Caffeine 1,20 6,00