Trypsin And Hydrochloric Acid Experiment

DISCUSSION:
The results obtained based off trials one, two, and three would support the initial rationale hypothesis that if salt concentration is not equal to the optimum concentration of 0.15M, then its ability to function will decrease. 0.15M with the trypsin added had the lowest absorbency compared to the three other concentrated groups that were tested (0.0M, 0.075M, 0.30M). Closer comparison that if you take the results obtained from trial one with trypsin at a salt concentration of 0.075M, the absorbance was 0.165 (440 nm wavelength). In comparison to 0.15M, which had an absorbency of 0.108 (440 nm wavelength). This would cause the enzyme to slow down and not work as effectively. Though the errors in the data would cause the hypothesis to be deemed inconclusive, the results obtained would lead to a rationale hypothesis that would be supported. It would not be due to chance, even with the errors because even with the errors obtained, every absorbency was higher than that of the optimum salt concentration of 0.15M.
Results were in comparison to the lab group on Wednesday. Though their group tested different salt concentration levels: (0.0M NaCl,0.15M NaCl,0.6M NaCl,1.2M NaC ,4.8M NaCl). Their rationale hypothesis “An increase of sodium chloride (NaCl) will decrease the proteolytic activity of trypsin based on the effect salt concentration has on protein denaturation” was proven throughout their experiment to be accepted (Theo Balog, Samantha Lin, Derek Margulies, Kayla McCulloch, Group Five: