Trypsin Lab Report
INTRODUCTION:
An enzyme is a substance that in most situations, is a catalyst, which increases the rate of reaction by contributing alternative reaction pathways of lower activation energy. Enzymes are able to do this by binding to reactant molecules and allowing them to cooperate in a more energy efficient way. If catalyzed by the appropriate enzyme, reactions that would take an extreme amount of time would rather occur in fractions of a second. First, enzymes increase the rate at which chemical reactions occur without themselves being permanently altered by the reaction. Then, they increase the rate of reaction without adjusting the equilibrium between reactants and products. Throughout this process, water-soluble vitamins act as coenzymes to help catalyze chemical reactions. Hence, enzymes react by compressing the activation energy, thereby …show more content…
increasing the rate of reaction (Cooper, 2000).
Enzymes are of importance in living systems for many different reasons. These globular proteins control the speed of chemical reactions and without them, these reactions would occur too slowly to keep the human body alive. Enzymes also allow for communication between cells, keeping growth, life, and death under control (Science Museum, N.D.).
The digestive enzyme lactase is used to break down lactose, a sugar found in many types of dairy products. Your body can’t automatically absorb lactose, so this enzyme is used to digest the sugar. Enzyme is being used as a catalyst when breaking down lactose into smaller, digestible sugars called glucose and galactose. On the other hand, the body can develop a condition known as lactose intolerance and this is present when not enough lactase is produced to function properly (Baseline of Health Foundation, 2017).
The task of this experiment was to determine how varying salt concentrations will affect the functionality of Trypsin, a proteolytic enzyme located in the small intestine.
It is logical that if the enzyme, Trypsin, functions at the optimum salt concentration of 0.15M, then its ability to function efficiently will decrease. The rationale for this proposed relationship is that Trypsin won’t function in a different concentration than 0.15M because it performs best at that value. Anywhere else it may break down or work at a slower pace.
MATERIALS & METHODS:
To better understand the effect of salt concentration on trypsin the tubes were marked as 2mL with the different salt concentrations. The first for tubes were negative control, the next four tubes were the first trial of trypsin, and the last four tubes were the second trial of trypsin. This step was done to differentiate between the tubes and make sure the solutions are put in the correct place. The micropipette that dials 1000 was set to 045 and 450µL of 0.0M NaCl(pH8) was measured and repeated three times and added to the correct tube. This step was repeated
for
An enzyme is a substance that in most situations, is a catalyst, which increases the rate of reaction by contributing alternative reaction pathways of lower activation energy. Enzymes are able to do this by binding to reactant molecules and allowing them to cooperate in a more energy efficient way. If catalyzed by the appropriate enzyme, reactions that would take an extreme amount of time would rather occur in fractions of a second. First, enzymes increase the rate at which chemical reactions occur without themselves being permanently altered by the reaction. Then, they increase the rate of reaction without adjusting the equilibrium between reactants and products. Throughout this process, water-soluble vitamins act as coenzymes to help catalyze chemical reactions. Hence, enzymes react by compressing the activation energy, thereby …show more content…
increasing the rate of reaction (Cooper, 2000).
Enzymes are of importance in living systems for many different reasons. These globular proteins control the speed of chemical reactions and without them, these reactions would occur too slowly to keep the human body alive. Enzymes also allow for communication between cells, keeping growth, life, and death under control (Science Museum, N.D.).
The digestive enzyme lactase is used to break down lactose, a sugar found in many types of dairy products. Your body can’t automatically absorb lactose, so this enzyme is used to digest the sugar. Enzyme is being used as a catalyst when breaking down lactose into smaller, digestible sugars called glucose and galactose. On the other hand, the body can develop a condition known as lactose intolerance and this is present when not enough lactase is produced to function properly (Baseline of Health Foundation, 2017).
The task of this experiment was to determine how varying salt concentrations will affect the functionality of Trypsin, a proteolytic enzyme located in the small intestine.
It is logical that if the enzyme, Trypsin, functions at the optimum salt concentration of 0.15M, then its ability to function efficiently will decrease. The rationale for this proposed relationship is that Trypsin won’t function in a different concentration than 0.15M because it performs best at that value. Anywhere else it may break down or work at a slower pace.
MATERIALS & METHODS:
To better understand the effect of salt concentration on trypsin the tubes were marked as 2mL with the different salt concentrations. The first for tubes were negative control, the next four tubes were the first trial of trypsin, and the last four tubes were the second trial of trypsin. This step was done to differentiate between the tubes and make sure the solutions are put in the correct place. The micropipette that dials 1000 was set to 045 and 450µL of 0.0M NaCl(pH8) was measured and repeated three times and added to the correct tube. This step was repeated
for