Understanding Nutrition
بسم الله الرحمن الرحيم
Al-Quds University
Body Fluid Lab. Report
Chemical Examination of Urine
Prepared By :
Lucia
Principles
Benedict's Test for Reducing Sugars
Urinary sugars when boiled in Benedict's reagent reduce copper sulphate to a reddish cuprous oxide precipitate in hot alkaline medium, the intensity of which is proportional to the amount of sugar present in the urine. The results are reported as 1+,2+, etc. depending upon the colour and intensity of the cuprous oxide precipitate.
turbidimetric method protein in urine
Quantitative Tests for 24-Hour Specimens.Trichloroacetic acid (TCA) test. The addition of TCA to a urine specimen precipitates the protein in a fine suspension that is quantified spectrophotometrically at 420 nm (nanometers) by comparison with a similarly treated standard.
Heat and acetic acid method Heat test: Based on the principle of heat coagulation and precipitation of proteins. If any turbidity appears, add 2 drops of 33% acetic acid. (Acidification is necessary because in alkaline medium heating may precipitate phosphates). If the precipitate is due to proteins, it will increase on acidification and if it is due to phosphates, it will dissolve again.
Sulphosalicylic acid method
Urine Protein Sulfosalicylic Acid Precipitation Test (SSA) Principle: Three percent (3%) Sulfosalicylic Acid (SSA reagent) is added to a small and equal volume of clear urine. The acidification causes precipitation of protein in the sample (seen as increasing turbidity), which is subjectively graded as trace,1+, 2+, 3+ or 4+.
Bence Jones protein
Bence Jones reaction involves heating urine to 140°F (60°C). At this temperature, the Bence Jones proteins will clump. The clumping disappears if the urine is further heated to boiling and reappears when the urine is cooled. Other clumping procedures using salts, acids, and other chemicals are also used to detect these proteins. These types of test will reveal whether or not
Al-Quds University
Body Fluid Lab. Report
Chemical Examination of Urine
Prepared By :
Lucia
Principles
Benedict's Test for Reducing Sugars
Urinary sugars when boiled in Benedict's reagent reduce copper sulphate to a reddish cuprous oxide precipitate in hot alkaline medium, the intensity of which is proportional to the amount of sugar present in the urine. The results are reported as 1+,2+, etc. depending upon the colour and intensity of the cuprous oxide precipitate.
turbidimetric method protein in urine
Quantitative Tests for 24-Hour Specimens.Trichloroacetic acid (TCA) test. The addition of TCA to a urine specimen precipitates the protein in a fine suspension that is quantified spectrophotometrically at 420 nm (nanometers) by comparison with a similarly treated standard.
Heat and acetic acid method Heat test: Based on the principle of heat coagulation and precipitation of proteins. If any turbidity appears, add 2 drops of 33% acetic acid. (Acidification is necessary because in alkaline medium heating may precipitate phosphates). If the precipitate is due to proteins, it will increase on acidification and if it is due to phosphates, it will dissolve again.
Sulphosalicylic acid method
Urine Protein Sulfosalicylic Acid Precipitation Test (SSA) Principle: Three percent (3%) Sulfosalicylic Acid (SSA reagent) is added to a small and equal volume of clear urine. The acidification causes precipitation of protein in the sample (seen as increasing turbidity), which is subjectively graded as trace,1+, 2+, 3+ or 4+.
Bence Jones protein
Bence Jones reaction involves heating urine to 140°F (60°C). At this temperature, the Bence Jones proteins will clump. The clumping disappears if the urine is further heated to boiling and reappears when the urine is cooled. Other clumping procedures using salts, acids, and other chemicals are also used to detect these proteins. These types of test will reveal whether or not