Leaf area of five randomly selected plants, was measured with the help of leaf area meter (Model LI-3100, USA) at 20, 40 and 60 DAS by destructive sampling from all leaves of the five sample plants (Sticker et al., 1961). Plants were cut at the soil surface, leaves were separated from the plants, covered with blotting paper to keep the leaf intact and retain its original shape and were brought to the laboratory. Leaf area was measured immediately after bringing them in the laboratory. Leaf area index was calculated as the ratio of leaf area to the ground area occupied by the plant (Diwaker and Oswalt, 1992).
Leaf area index = Total leaf area (cm2) ¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬ Ground area covered (cm2)
3.8.1.3. Dry matter accumulation
Ten randomly selected plants from sampled row were cut at the soil surface at 20, 40, 60 DAS and at …show more content…
Accordingly, the easily oxidizable portion of the 10 g soil organic matter was oxidized using alkaline potassium permanganate solution and the evolved ammonia was distilled to boric acid, then the ammonia trapped in boric acid was quantified using 0.02 N H2SO4.
3.10.1.4. Available P
The available P content in soil was estimated by Olsen’s method (Olsen et al., 1954). The soil samples (5 g) were extracted with 0.5M NaHCO3 at pH 8.5. The P content in the soil extract was determined by the blue color formed by ascorbic acid-molybdate complex and the color intensity was read at 660 nm using spectrophotometer as outlined by Olsen et al. (1954).
3.10.1.5. Available potassium
Available K was determined using neutral normal ammonium acetate extraction method and flame photometry as described by Jackson, 1973 and expressed in kg/ha.
3.10.2. Soil biological