Yuguo Hou Glucose Intake Lab Report
Name: Yuguo Hou Glucose Intake Experiment
In ruminants, starch is enzymatic hydrolyzed digested to glucose which is highly energetically efficient and absorbed by brush border membrane in small intestinal. In order to better understanding of digestion and absorption of glucose in the ruminants, they did a lot of experiments on three kinds of ruminate, cow, calf and sheep.
Determining from different aspects such as mRNA expression, protein expression and enzyme activity, hormone content, glucose intake and histological examination. After RNA extraction, the PCR application, gel purified and ligated with pGEM-T Easy vector, they successfully cloned and sequenced the taste receptors from ovine tongue and intestine tissues. Immunohistory was applied on sheep small intestinal tissues by using the primary antibodies to T1R2, T1R3, GLP-2, a-gustducin, and the housekeeping maker of enteroendocrine cells chromogranin-A, revealed that T1R2 and T1R3 are expressed in L-endocrine cells. …show more content…
In bovine, quantitative real-time reverse transcription PCR was used to determine the relative mRNA expression in the intestine of calves maintained on different diets, also to test if the artificial sweetener enhances SGLT1 expression and mucosal growth in ruminant animals. Brush border membrane vesicles were isolated from calf duodenal tissues, histological and morphometric analysis was used to visualize villus height and crypt depth the response to supplementation with the artificial sweetener Sucram. The protein abundance of bovine duodenum sodium-glucose co-transporter 1 and b-actin were performed by Western blotting. Radioactive (U-14C)-d-glucose was used to measure the glucose uptake. Increasing enzymes activities of maltase and alkaline phosphatase in intestine was test by enzyme activity
In ruminants, starch is enzymatic hydrolyzed digested to glucose which is highly energetically efficient and absorbed by brush border membrane in small intestinal. In order to better understanding of digestion and absorption of glucose in the ruminants, they did a lot of experiments on three kinds of ruminate, cow, calf and sheep.
Determining from different aspects such as mRNA expression, protein expression and enzyme activity, hormone content, glucose intake and histological examination. After RNA extraction, the PCR application, gel purified and ligated with pGEM-T Easy vector, they successfully cloned and sequenced the taste receptors from ovine tongue and intestine tissues. Immunohistory was applied on sheep small intestinal tissues by using the primary antibodies to T1R2, T1R3, GLP-2, a-gustducin, and the housekeeping maker of enteroendocrine cells chromogranin-A, revealed that T1R2 and T1R3 are expressed in L-endocrine cells. …show more content…
In bovine, quantitative real-time reverse transcription PCR was used to determine the relative mRNA expression in the intestine of calves maintained on different diets, also to test if the artificial sweetener enhances SGLT1 expression and mucosal growth in ruminant animals. Brush border membrane vesicles were isolated from calf duodenal tissues, histological and morphometric analysis was used to visualize villus height and crypt depth the response to supplementation with the artificial sweetener Sucram. The protein abundance of bovine duodenum sodium-glucose co-transporter 1 and b-actin were performed by Western blotting. Radioactive (U-14C)-d-glucose was used to measure the glucose uptake. Increasing enzymes activities of maltase and alkaline phosphatase in intestine was test by enzyme activity