Chem-102 Lab Dr. Sobhi Experiment #3: Spectrophotometric Determination of Tartrazine Purpose The objectives of this lab include- illustrating the use of the spectrophotometer in chemical analysis‚ and generating a standard‚ or calibration curve‚ then using that curve to determine the value of an unknown substance. The spectrophotometer is one of the most powerful tools used in chemistry to find the concentration of substances in solution. It compares the colors of a known and an unknown solution
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Crystallinity of SAPO-5 With Molar Composition The given mixture composition (Al: 1.3 P: 0.8Si: 1.2TEA: 100H2O ; Al: 1.3P : 0.05Si: 1.2TEA: 100H2O ; Al: 1.3P : 0.05Si: 0.2TEA: 100H2O) is used to study the overall effect of silicon in AFI framework.The SiO2 magnitude is varied mole ratio from 0 to 1.0 of the given SiO2 to Al2 .The crystals SEM images that is obtained by using various contents of silicon are representing the sample produced in the absence of silicon in reactant gel is similar to the
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Implementation of a Chirped Pulse Fourier Transform Microwave (CP-FTMW) Spectrometer Abstract: Under the condition in which electric and magnetic fields are absent‚ rotational constants is a three molecular constants that substantiate polyatomic molecule. In this laboratory experiment at Missouri University of Science and Technology‚ we are going to collect rotational spectra by using 2 separate types of microwave spectrometers -- the FTMW (Fourier Transform Microwave) spectrometer and CP-FTMW
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Based on the data from the riboflavin spectrum scan‚ the maximum absorbance wavelength for this compound is 446 nm. This was the point between 390 nm and 500 nm at which the absorbance value (0.72) was the highest. A blank tube that has the components of the solution being examined except for the compound of interest is then used in combination to provide an even more accurate reading. Then‚ by using Beer’s Law‚ the molar extinction coefficient for riboflavin was able to be calculated: 14‚400 L/(moles*cm)
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Absorption & Emission of Light Stimulated Absorption & Emission‚ Spontaneous Emission E2 Incident radiation hν hν Emitted & transmitted radiation E1 Resonance condition E2-E1=hν • Rate of stimulated emission is: −dN1/dt = N1B1->2 ρ(ν) • Rate of absorption is the sum of the rated due to stimulated emission and spontaneous emission: −dN2/dt = N2B2->1 ρ(ν) + N2A2->1 where B1->2 is the Einstein transition probability for induced absorption‚ B2->1 is the Einstein transition probability
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Absorption and marginal costing (Relevant to AAT Examination Paper 3: Management Accounting) Li Tak Ming‚ Andy Deputy Head‚ Department of Business Administration‚ Hong Kong Institute of Vocational Education (Kwai Chung) Introduction Absorption costing and marginal costing are alternative cost accumulation systems used to ascertain product or job costs for inventory valuation and cost of sales. Absorption costing Absorption costing includes both variable and fixed production costs in the
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ESTIMATION OF IRON IN IRON ORE-SPECTROPHOTOMETRIC METHOD By: Taylor Villari Experiment conducted on 7/22/13 Components of each test tube examined in the spectrophotometer Trial | Volume of Iron solution (mL) | Micrograms of Iron | Volume of 10% sodium acetate | Volume of 0.1% o-phenanthroline | Volume of water (mL) | 1(blank) | 0.0 | 0.0 | 1.0 mL | 1.0 mL | 8.0 mL | 2 | 1.0 mL | 10 | 1.0 mL | 1.0 mL | 7.0 mL | 3 | 3.0 mL | 30 | 1.0 mL | 1.0 mL | 5.0 mL | 4 | 5.0 mL | 50 | 1.0 mL
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Extraction and Quantitative Determination of B-Carotene Introduction The study of B-Carotene is important because it is good for vision‚ immunity‚ and overall health. The purpose of this experiment is to extract and find quantitative amount of Beta-carotene from a carrot sample. The raw product(carrots) was extracted using simple distillation. The purity of the product will be characterized using percent yield‚ thin layer chromatography‚ Beer’s Law‚ and UV-vis. Beer’s law is used because in this
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In experiment 3.1.We set up and calibrate the spectronic 20 first since it takes time to be ready to use so‚ we turned the spectronic 20 to warm up for at least 15 minutes‚ set wavelength to 360 while no test tube in it. Also‚ we set the filter lever in order to match the wavelength range‚ and then set the transmittance to 0%‚ then we changed the mode to absorbance. After we labeled two test tubes which were #5 and #10‚ we inserted water first to set 0 absorbance‚ then we used #5 sample to read it
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1. The standard curve is a graph that shows a relationship between concentration and absorbance of a solution. A standard curve was experimentally created in this experiment using 10mL solutions of phenol red with concentrations 10µM‚ 7.5 µM‚ 5.0 µM and 2.5 µM then the absorbance of each sample was measured using a spectrophotometer. This generated curve with resulting average absorbances of 1.273nm‚ 1.0275nm‚ 0.585nm‚ 0.285nm and 0.124nm provided a means to determine the phenol red concentrations
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